Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12120 | 3' | -53.6 | NC_003309.1 | + | 15478 | 0.66 | 0.827978 |
Target: 5'- uUCGGGucCGCCGUCGGAUugccggCCgGAAGUu -3' miRNA: 3'- -AGCCUcuGUGGCGGCUUA------GGgUUUCG- -5' |
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12120 | 3' | -53.6 | NC_003309.1 | + | 35065 | 0.66 | 0.827978 |
Target: 5'- aCGGcGACGUCGCaCcGAUCCCGaccGAGCg -3' miRNA: 3'- aGCCuCUGUGGCG-GcUUAGGGU---UUCG- -5' |
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12120 | 3' | -53.6 | NC_003309.1 | + | 4314 | 0.66 | 0.827978 |
Target: 5'- gUUGGcgauGACGCCGaUCGAcccuacgCCCGAAGUg -3' miRNA: 3'- -AGCCu---CUGUGGC-GGCUua-----GGGUUUCG- -5' |
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12120 | 3' | -53.6 | NC_003309.1 | + | 5252 | 0.66 | 0.818748 |
Target: 5'- cUCGGgcaccaGGACGCCGCCagcaCCC--GGCg -3' miRNA: 3'- -AGCC------UCUGUGGCGGcuuaGGGuuUCG- -5' |
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12120 | 3' | -53.6 | NC_003309.1 | + | 2880 | 0.66 | 0.795803 |
Target: 5'- gCGGGuaaaguccuugaauGACGCCGuCCGAAUCgCGAucgaugaAGCu -3' miRNA: 3'- aGCCU--------------CUGUGGC-GGCUUAGgGUU-------UCG- -5' |
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12120 | 3' | -53.6 | NC_003309.1 | + | 18700 | 0.66 | 0.789905 |
Target: 5'- gCGGAuACgGCCGCCGA-UCCgGcGGCc -3' miRNA: 3'- aGCCUcUG-UGGCGGCUuAGGgUuUCG- -5' |
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12120 | 3' | -53.6 | NC_003309.1 | + | 41412 | 0.67 | 0.769824 |
Target: 5'- gCGGcaugcgcaAGGCACCGCCcgucGAAUCgaaGAAGCg -3' miRNA: 3'- aGCC--------UCUGUGGCGG----CUUAGgg-UUUCG- -5' |
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12120 | 3' | -53.6 | NC_003309.1 | + | 51416 | 0.67 | 0.759562 |
Target: 5'- cUGGAaACAUCGCCGGG--CCAAAGUc -3' miRNA: 3'- aGCCUcUGUGGCGGCUUagGGUUUCG- -5' |
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12120 | 3' | -53.6 | NC_003309.1 | + | 543 | 0.67 | 0.758528 |
Target: 5'- uUCGGcuuugcgccgaccAGGCGCUGCCGGcUCgCCGggucGAGCc -3' miRNA: 3'- -AGCC-------------UCUGUGGCGGCUuAG-GGU----UUCG- -5' |
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12120 | 3' | -53.6 | NC_003309.1 | + | 23617 | 0.67 | 0.749168 |
Target: 5'- cCGGcGaACACgCGCCGGAUCUCcggauggucguuGAGGCu -3' miRNA: 3'- aGCCuC-UGUG-GCGGCUUAGGG------------UUUCG- -5' |
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12120 | 3' | -53.6 | NC_003309.1 | + | 7208 | 0.67 | 0.728028 |
Target: 5'- cCGGcAG-UACCGCCGugauGUCgUAAAGCc -3' miRNA: 3'- aGCC-UCuGUGGCGGCu---UAGgGUUUCG- -5' |
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12120 | 3' | -53.6 | NC_003309.1 | + | 34309 | 0.68 | 0.717306 |
Target: 5'- gCGGAGcucgACCGCCGuuucaAGUCCUGAAGg -3' miRNA: 3'- aGCCUCug--UGGCGGC-----UUAGGGUUUCg -5' |
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12120 | 3' | -53.6 | NC_003309.1 | + | 32187 | 0.68 | 0.7065 |
Target: 5'- gUCGccGACGCCGCCcgcaCCCAagaGAGCa -3' miRNA: 3'- -AGCcuCUGUGGCGGcuuaGGGU---UUCG- -5' |
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12120 | 3' | -53.6 | NC_003309.1 | + | 26043 | 0.69 | 0.607255 |
Target: 5'- gUCGGAGcagucACAgUCGCCGGggCCCAuAGUc -3' miRNA: 3'- -AGCCUC-----UGU-GGCGGCUuaGGGUuUCG- -5' |
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12120 | 3' | -53.6 | NC_003309.1 | + | 41032 | 0.71 | 0.513816 |
Target: 5'- gUCGG-GACGCCGCCGcugcacaagcgcgacAGcgCCCAAcAGCu -3' miRNA: 3'- -AGCCuCUGUGGCGGC---------------UUa-GGGUU-UCG- -5' |
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12120 | 3' | -53.6 | NC_003309.1 | + | 43914 | 0.76 | 0.284919 |
Target: 5'- gUCGGAGAUACCaGCCGGcAUCCUgcaauGCa -3' miRNA: 3'- -AGCCUCUGUGG-CGGCU-UAGGGuuu--CG- -5' |
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12120 | 3' | -53.6 | NC_003309.1 | + | 12923 | 1.13 | 0.00076 |
Target: 5'- aUCGGAGACACCGCCGAAUCCCAAAGCg -3' miRNA: 3'- -AGCCUCUGUGGCGGCUUAGGGUUUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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