Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12120 | 5' | -53.9 | NC_003309.1 | + | 3153 | 0.66 | 0.815469 |
Target: 5'- cUUGgUCCuuGAGCGCC-GGGGCAUCcuucgGCCg -3' miRNA: 3'- -AGCgAGG--CUUGUGGaCUCUGUAG-----CGG- -5' |
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12120 | 5' | -53.9 | NC_003309.1 | + | 43747 | 0.66 | 0.815469 |
Target: 5'- cCGC-CCGAugagGCAU---AGGCAUCGCCc -3' miRNA: 3'- aGCGaGGCU----UGUGgacUCUGUAGCGG- -5' |
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12120 | 5' | -53.9 | NC_003309.1 | + | 5563 | 0.66 | 0.796347 |
Target: 5'- uUCGCgcgcgCCGAucGCGgCgGuGAgGUCGCCg -3' miRNA: 3'- -AGCGa----GGCU--UGUgGaCuCUgUAGCGG- -5' |
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12120 | 5' | -53.9 | NC_003309.1 | + | 8507 | 0.66 | 0.796347 |
Target: 5'- cCGCUCacacGCACgCUGu-ACGUCGCCg -3' miRNA: 3'- aGCGAGgcu-UGUG-GACucUGUAGCGG- -5' |
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12120 | 5' | -53.9 | NC_003309.1 | + | 48235 | 0.66 | 0.776533 |
Target: 5'- cUCGCucgaucgagccgUCCGAGCGCCgcuGACG-CGCg -3' miRNA: 3'- -AGCG------------AGGCUUGUGGacuCUGUaGCGg -5' |
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12120 | 5' | -53.9 | NC_003309.1 | + | 10951 | 0.67 | 0.756116 |
Target: 5'- aCGCg-CGAGCGCCgucUGcGcCGUCGCCg -3' miRNA: 3'- aGCGagGCUUGUGG---ACuCuGUAGCGG- -5' |
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12120 | 5' | -53.9 | NC_003309.1 | + | 30546 | 0.67 | 0.74571 |
Target: 5'- aUCGUaguaugaaCGAACGCCUGAuGACAcCgGCCg -3' miRNA: 3'- -AGCGag------GCUUGUGGACU-CUGUaG-CGG- -5' |
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12120 | 5' | -53.9 | NC_003309.1 | + | 1876 | 0.67 | 0.744662 |
Target: 5'- cCGUUUCGAGCAaacagcCCUGAuugacccacgccuGAUAUCGCUc -3' miRNA: 3'- aGCGAGGCUUGU------GGACU-------------CUGUAGCGG- -5' |
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12120 | 5' | -53.9 | NC_003309.1 | + | 33011 | 0.67 | 0.735187 |
Target: 5'- aUCGUUCCG-ACACUUGccGCAUcCGCa -3' miRNA: 3'- -AGCGAGGCuUGUGGACucUGUA-GCGg -5' |
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12120 | 5' | -53.9 | NC_003309.1 | + | 30157 | 0.67 | 0.735187 |
Target: 5'- aCGCgaacgUCCGGACGgCgcagGGGAUAUgCGCCc -3' miRNA: 3'- aGCG-----AGGCUUGUgGa---CUCUGUA-GCGG- -5' |
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12120 | 5' | -53.9 | NC_003309.1 | + | 15152 | 0.67 | 0.72456 |
Target: 5'- -gGCUCC-AGCugCUG-GAUGUCuGCCg -3' miRNA: 3'- agCGAGGcUUGugGACuCUGUAG-CGG- -5' |
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12120 | 5' | -53.9 | NC_003309.1 | + | 43212 | 0.67 | 0.72456 |
Target: 5'- aCGCUUCGAGCAUCcGAu-CGUCGUUg -3' miRNA: 3'- aGCGAGGCUUGUGGaCUcuGUAGCGG- -5' |
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12120 | 5' | -53.9 | NC_003309.1 | + | 47636 | 0.68 | 0.707369 |
Target: 5'- gCGCUCUugugccaaucggaagGAACGuCCUGcccGGuCGUCGCCg -3' miRNA: 3'- aGCGAGG---------------CUUGU-GGAC---UCuGUAGCGG- -5' |
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12120 | 5' | -53.9 | NC_003309.1 | + | 35057 | 0.68 | 0.70304 |
Target: 5'- gUCGCaccgaucccgaCCGAGCGCCUGuG-CGaCGCCg -3' miRNA: 3'- -AGCGa----------GGCUUGUGGACuCuGUaGCGG- -5' |
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12120 | 5' | -53.9 | NC_003309.1 | + | 15414 | 0.68 | 0.701956 |
Target: 5'- gCGC-CgGAACACCUucgcgccGAcGAgAUCGCCa -3' miRNA: 3'- aGCGaGgCUUGUGGA-------CU-CUgUAGCGG- -5' |
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12120 | 5' | -53.9 | NC_003309.1 | + | 5267 | 0.69 | 0.648213 |
Target: 5'- cUCGCUCgacagguucuCGGGCACCa-GGACGcCGCCa -3' miRNA: 3'- -AGCGAG----------GCUUGUGGacUCUGUaGCGG- -5' |
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12120 | 5' | -53.9 | NC_003309.1 | + | 14204 | 0.69 | 0.593001 |
Target: 5'- gUCGUUCUGAGcCGCCgcgaAGAgAUCGCUa -3' miRNA: 3'- -AGCGAGGCUU-GUGGac--UCUgUAGCGG- -5' |
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12120 | 5' | -53.9 | NC_003309.1 | + | 20541 | 0.69 | 0.593001 |
Target: 5'- gUCGUUCCGucgaGCaUGAGGCG-CGCCg -3' miRNA: 3'- -AGCGAGGCuug-UGgACUCUGUaGCGG- -5' |
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12120 | 5' | -53.9 | NC_003309.1 | + | 20177 | 0.7 | 0.549369 |
Target: 5'- uUCGCcgacaCGAGCACCUGcgauucGACGaCGCCg -3' miRNA: 3'- -AGCGag---GCUUGUGGACu-----CUGUaGCGG- -5' |
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12120 | 5' | -53.9 | NC_003309.1 | + | 6252 | 0.72 | 0.465666 |
Target: 5'- cUCGUcgUCGAAC-CCUGcGAUGUCGCCu -3' miRNA: 3'- -AGCGa-GGCUUGuGGACuCUGUAGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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