Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12122 | 3' | -65 | NC_003309.1 | + | 47732 | 0.66 | 0.290985 |
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Target: 5'- cCGGCCGCcUUGACGACGCGcGCc--- -3' miRNA: 3'- -GCCGGCGuGGCUGCUGCGCcCGcccu -5' |
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| 12122 | 3' | -65 | NC_003309.1 | + | 31804 | 0.66 | 0.277685 |
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Target: 5'- uGGCUGCuACUGACGAaGCGaaucggaaacGcGCGGGAu -3' miRNA: 3'- gCCGGCG-UGGCUGCUgCGC----------C-CGCCCU- -5' |
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| 12122 | 3' | -65 | NC_003309.1 | + | 15717 | 0.67 | 0.258651 |
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Target: 5'- -uGCUGCAUCGAuCGAgCGCGGGaucgcucaCGGGGu -3' miRNA: 3'- gcCGGCGUGGCU-GCU-GCGCCC--------GCCCU- -5' |
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| 12122 | 3' | -65 | NC_003309.1 | + | 25865 | 0.67 | 0.240115 |
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Target: 5'- uCGGg-GCGCCGAacagcacCGGCGCGaGCGGGAu -3' miRNA: 3'- -GCCggCGUGGCU-------GCUGCGCcCGCCCU- -5' |
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| 12122 | 3' | -65 | NC_003309.1 | + | 53308 | 0.68 | 0.218386 |
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Target: 5'- -cGCCGgGCgGGCaACGaCGGGCGGGc -3' miRNA: 3'- gcCGGCgUGgCUGcUGC-GCCCGCCCu -5' |
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| 12122 | 3' | -65 | NC_003309.1 | + | 32906 | 0.69 | 0.183601 |
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Target: 5'- gCGGCCGgACUGcGCGACGgcaGGGUucucGGGAc -3' miRNA: 3'- -GCCGGCgUGGC-UGCUGCg--CCCG----CCCU- -5' |
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| 12122 | 3' | -65 | NC_003309.1 | + | 22486 | 0.7 | 0.146132 |
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Target: 5'- aCGGCgGcCACCGcCGACGCGGcggcaucgugcGCGGcGAu -3' miRNA: 3'- -GCCGgC-GUGGCuGCUGCGCC-----------CGCC-CU- -5' |
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| 12122 | 3' | -65 | NC_003309.1 | + | 13980 | 1.08 | 0.000185 |
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Target: 5'- uCGGCCGCACCGACGACGCGGGCGGGAg -3' miRNA: 3'- -GCCGGCGUGGCUGCUGCGCCCGCCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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