Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12123 | 3' | -55.9 | NC_003309.1 | + | 5610 | 0.67 | 0.636291 |
Target: 5'- cCCUUCGgggUGUUCgccGuGCCGUcguCGCGAAu -3' miRNA: 3'- -GGAAGCa--GCAAGa--CuCGGCG---GCGCUU- -5' |
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12123 | 3' | -55.9 | NC_003309.1 | + | 5154 | 0.67 | 0.614354 |
Target: 5'- gCUgcgCGUCGccgCgc-GCCGCCGCGAGc -3' miRNA: 3'- gGAa--GCAGCaa-GacuCGGCGGCGCUU- -5' |
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12123 | 3' | -55.9 | NC_003309.1 | + | 45531 | 0.67 | 0.614354 |
Target: 5'- ----gGUCGUaUUGAcgGCCGCCGCGGc -3' miRNA: 3'- ggaagCAGCAaGACU--CGGCGGCGCUu -5' |
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12123 | 3' | -55.9 | NC_003309.1 | + | 12077 | 0.69 | 0.51738 |
Target: 5'- cCCUUCGcuaCGc---GGGCCGCCGCGGc -3' miRNA: 3'- -GGAAGCa--GCaagaCUCGGCGGCGCUu -5' |
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12123 | 3' | -55.9 | NC_003309.1 | + | 8344 | 0.71 | 0.381127 |
Target: 5'- cCCUUCGUCGUUCgacugGuaauuGCCGUCGacCGAGa -3' miRNA: 3'- -GGAAGCAGCAAGa----Cu----CGGCGGC--GCUU- -5' |
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12123 | 3' | -55.9 | NC_003309.1 | + | 2481 | 0.73 | 0.299605 |
Target: 5'- uCCUUCGcugaacgacUCGcgCagcgcGAGCCGCCGCGAGa -3' miRNA: 3'- -GGAAGC---------AGCaaGa----CUCGGCGGCGCUU- -5' |
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12123 | 3' | -55.9 | NC_003309.1 | + | 14210 | 1.08 | 0.000999 |
Target: 5'- cCCUUCGUCGUUCUGAGCCGCCGCGAAg -3' miRNA: 3'- -GGAAGCAGCAAGACUCGGCGGCGCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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