Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12123 | 5' | -59.4 | NC_003309.1 | + | 31426 | 0.66 | 0.480625 |
Target: 5'- cGCUCGCuGGcgacCAGUgcgcaGCCGCCGGCGu -3' miRNA: 3'- aUGGGUG-CCa---GUCGaa---CGGCGGCUGC- -5' |
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12123 | 5' | -59.4 | NC_003309.1 | + | 31925 | 0.66 | 0.46971 |
Target: 5'- cGCCCGCGGcCGGCgccagcgcgucuuUUGCuCGUCGcuuGCGg -3' miRNA: 3'- aUGGGUGCCaGUCG-------------AACG-GCGGC---UGC- -5' |
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12123 | 5' | -59.4 | NC_003309.1 | + | 22998 | 0.66 | 0.451163 |
Target: 5'- -gUCaGCGaGUCGacGCUUGCCGCCGAUc -3' miRNA: 3'- auGGgUGC-CAGU--CGAACGGCGGCUGc -5' |
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12123 | 5' | -59.4 | NC_003309.1 | + | 5555 | 0.66 | 0.480625 |
Target: 5'- cGCCgauCGCGG-CGGUgagGUCGCCGACc -3' miRNA: 3'- aUGG---GUGCCaGUCGaa-CGGCGGCUGc -5' |
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12123 | 5' | -59.4 | NC_003309.1 | + | 15339 | 0.67 | 0.432081 |
Target: 5'- cACCCGCaGcGUCGGCgaugGCUGCUGcGCGu -3' miRNA: 3'- aUGGGUG-C-CAGUCGaa--CGGCGGC-UGC- -5' |
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12123 | 5' | -59.4 | NC_003309.1 | + | 5070 | 0.67 | 0.432081 |
Target: 5'- cAUgCACGGgcgCGGCggccggUGCCGCgaCGGCGg -3' miRNA: 3'- aUGgGUGCCa--GUCGa-----ACGGCG--GCUGC- -5' |
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12123 | 5' | -59.4 | NC_003309.1 | + | 22409 | 0.67 | 0.422719 |
Target: 5'- uUGCCCAaCGcuUCGGCaUGCCGCUGcGCGu -3' miRNA: 3'- -AUGGGU-GCc-AGUCGaACGGCGGC-UGC- -5' |
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12123 | 5' | -59.4 | NC_003309.1 | + | 39247 | 0.67 | 0.395379 |
Target: 5'- aGCCCGCGGaUCAGCaucgcGCacgaagGUCGACGa -3' miRNA: 3'- aUGGGUGCC-AGUCGaa---CGg-----CGGCUGC- -5' |
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12123 | 5' | -59.4 | NC_003309.1 | + | 5854 | 0.68 | 0.344248 |
Target: 5'- -uCCCAcCGGguagcccuUCAGCaUGCCGuuGGCGa -3' miRNA: 3'- auGGGU-GCC--------AGUCGaACGGCggCUGC- -5' |
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12123 | 5' | -59.4 | NC_003309.1 | + | 7051 | 0.69 | 0.336202 |
Target: 5'- gGCCCACGGUCGcGCaUGCaCGaCCcACGc -3' miRNA: 3'- aUGGGUGCCAGU-CGaACG-GC-GGcUGC- -5' |
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12123 | 5' | -59.4 | NC_003309.1 | + | 10021 | 0.7 | 0.27681 |
Target: 5'- uUACCgACGGUCAGCUUucccucuucgGCCaUCGACu -3' miRNA: 3'- -AUGGgUGCCAGUCGAA----------CGGcGGCUGc -5' |
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12123 | 5' | -59.4 | NC_003309.1 | + | 41299 | 0.71 | 0.23198 |
Target: 5'- cGCCUGCGGgaugggaaCGGCUUGaCGCUGGCGg -3' miRNA: 3'- aUGGGUGCCa-------GUCGAACgGCGGCUGC- -5' |
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12123 | 5' | -59.4 | NC_003309.1 | + | 6356 | 0.72 | 0.214161 |
Target: 5'- --gCCGCGGUgGGCUUGgcgcuuucgcccuUCGCCGACGc -3' miRNA: 3'- augGGUGCCAgUCGAAC-------------GGCGGCUGC- -5' |
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12123 | 5' | -59.4 | NC_003309.1 | + | 19392 | 0.73 | 0.178689 |
Target: 5'- aUGCCCgccGCGGUCAGCgacaCGaCCGACGg -3' miRNA: 3'- -AUGGG---UGCCAGUCGaacgGC-GGCUGC- -5' |
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12123 | 5' | -59.4 | NC_003309.1 | + | 44184 | 0.74 | 0.148129 |
Target: 5'- cGCCCGCGG-CGcGC-UGCCGCuCGGCGu -3' miRNA: 3'- aUGGGUGCCaGU-CGaACGGCG-GCUGC- -5' |
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12123 | 5' | -59.4 | NC_003309.1 | + | 16878 | 0.74 | 0.144173 |
Target: 5'- aGCCCGCcG-C-GCUUGCUGCCGACGa -3' miRNA: 3'- aUGGGUGcCaGuCGAACGGCGGCUGC- -5' |
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12123 | 5' | -59.4 | NC_003309.1 | + | 17522 | 0.75 | 0.122403 |
Target: 5'- cACCCACGGcgcgucggacgUCAGCg-GCaCGCCGACGc -3' miRNA: 3'- aUGGGUGCC-----------AGUCGaaCG-GCGGCUGC- -5' |
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12123 | 5' | -59.4 | NC_003309.1 | + | 14176 | 1.07 | 0.000511 |
Target: 5'- cUACCCACGGUCAGCUUGCCGCCGACGa -3' miRNA: 3'- -AUGGGUGCCAGUCGAACGGCGGCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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