Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12124 | 5' | -55.9 | NC_003309.1 | + | 14670 | 0.66 | 0.696159 |
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Target: 5'- gCGaCCGAGguGUUgCuGGAAAUCCgAa -3' miRNA: 3'- -GC-GGCUCguCAAgGcCCUUUAGGgUg -5' |
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| 12124 | 5' | -55.9 | NC_003309.1 | + | 38835 | 0.66 | 0.685312 |
|
Target: 5'- uCGCuCGAGC--UUUCGGGAuAAUCCuCGCc -3' miRNA: 3'- -GCG-GCUCGucAAGGCCCU-UUAGG-GUG- -5' |
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| 12124 | 5' | -55.9 | NC_003309.1 | + | 190 | 0.66 | 0.674414 |
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Target: 5'- aGCgCGcGCuug-CCGGGAuuUCCCGCc -3' miRNA: 3'- gCG-GCuCGucaaGGCCCUuuAGGGUG- -5' |
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| 12124 | 5' | -55.9 | NC_003309.1 | + | 43623 | 0.66 | 0.641524 |
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Target: 5'- gGCCGAucucGCGGUgcuugUCCGGGug--CCCGu -3' miRNA: 3'- gCGGCU----CGUCA-----AGGCCCuuuaGGGUg -5' |
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| 12124 | 5' | -55.9 | NC_003309.1 | + | 47540 | 0.69 | 0.460558 |
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Target: 5'- uCGUCGAuGCAGUccgCCGGGgcAUgCCCAa -3' miRNA: 3'- -GCGGCU-CGUCAa--GGCCCuuUA-GGGUg -5' |
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| 12124 | 5' | -55.9 | NC_003309.1 | + | 19441 | 0.71 | 0.367302 |
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Target: 5'- gGCgGAGCcGUcacCCGGGAAcgCCCAUu -3' miRNA: 3'- gCGgCUCGuCAa--GGCCCUUuaGGGUG- -5' |
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| 12124 | 5' | -55.9 | NC_003309.1 | + | 14904 | 1.08 | 0.000913 |
|
Target: 5'- uCGCCGAGCAGUUCCGGGAAAUCCCACc -3' miRNA: 3'- -GCGGCUCGUCAAGGCCCUUUAGGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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