Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12125 | 5' | -54.5 | NC_003309.1 | + | 16252 | 1.09 | 0.000984 |
Target: 5'- cGUGCACACCAUGCACGAAUGGGCGACc -3' miRNA: 3'- -CACGUGUGGUACGUGCUUACCCGCUG- -5' |
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12125 | 5' | -54.5 | NC_003309.1 | + | 41303 | 0.69 | 0.536821 |
Target: 5'- -cGCACGCC-UGCG-GGAUGGGaaCGGCu -3' miRNA: 3'- caCGUGUGGuACGUgCUUACCC--GCUG- -5' |
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12125 | 5' | -54.5 | NC_003309.1 | + | 12861 | 0.68 | 0.569814 |
Target: 5'- -cGCGCGCCGaaaaccGCGCGAGgucGGCGAUc -3' miRNA: 3'- caCGUGUGGUa-----CGUGCUUac-CCGCUG- -5' |
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12125 | 5' | -54.5 | NC_003309.1 | + | 9320 | 0.68 | 0.569814 |
Target: 5'- -cGCcuacuuCGCCAUGCGCaucaGGGCGGCu -3' miRNA: 3'- caCGu-----GUGGUACGUGcuuaCCCGCUG- -5' |
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12125 | 5' | -54.5 | NC_003309.1 | + | 42931 | 0.67 | 0.648216 |
Target: 5'- --cCGCGCCAUGCaggcGCGAucuacugcauUGGGCGAg -3' miRNA: 3'- cacGUGUGGUACG----UGCUu---------ACCCGCUg -5' |
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12125 | 5' | -54.5 | NC_003309.1 | + | 9510 | 0.67 | 0.648216 |
Target: 5'- -cGCGCACCGugaccUGCACGcuUGcGGCGu- -3' miRNA: 3'- caCGUGUGGU-----ACGUGCuuAC-CCGCug -5' |
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12125 | 5' | -54.5 | NC_003309.1 | + | 32968 | 0.67 | 0.636979 |
Target: 5'- -cGCGCGCUgagGCGCGAGaGcGCGACg -3' miRNA: 3'- caCGUGUGGua-CGUGCUUaCcCGCUG- -5' |
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12125 | 5' | -54.5 | NC_003309.1 | + | 52965 | 0.67 | 0.635855 |
Target: 5'- -aGCGCACCGcacccccggugcuUGCACGGcuucAUcGGGCGuCg -3' miRNA: 3'- caCGUGUGGU-------------ACGUGCU----UA-CCCGCuG- -5' |
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12125 | 5' | -54.5 | NC_003309.1 | + | 45222 | 0.66 | 0.736601 |
Target: 5'- -gGCACuCCA-GCGCaGAAUgGGGCGGu -3' miRNA: 3'- caCGUGuGGUaCGUG-CUUA-CCCGCUg -5' |
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12125 | 5' | -54.5 | NC_003309.1 | + | 36860 | 0.66 | 0.725809 |
Target: 5'- --cCACGCCcgGCucuccccuCGGAuaUGGGCGGCc -3' miRNA: 3'- cacGUGUGGuaCGu-------GCUU--ACCCGCUG- -5' |
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12125 | 5' | -54.5 | NC_003309.1 | + | 46638 | 0.66 | 0.690682 |
Target: 5'- -aGCGCACCGgccuccuccaacGUGCGAA-GGGuCGACg -3' miRNA: 3'- caCGUGUGGUa-----------CGUGCUUaCCC-GCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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