Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12126 | 3' | -54.1 | NC_003309.1 | + | 39494 | 0.66 | 0.729459 |
Target: 5'- --gAGCGCAGcaucauccucUACgCGgaaUCGUGCAGCGu -3' miRNA: 3'- guaUCGCGUC----------AUG-GC---AGCACGUCGUc -5' |
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12126 | 3' | -54.1 | NC_003309.1 | + | 16585 | 1.09 | 0.001206 |
Target: 5'- aCAUAGCGCAGUACCGUCGUGCAGCAGu -3' miRNA: 3'- -GUAUCGCGUCAUGGCAGCACGUCGUC- -5' |
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12126 | 3' | -54.1 | NC_003309.1 | + | 5359 | 0.66 | 0.761327 |
Target: 5'- --aGGCGCGGgauggugauguUGCCGuUCGagaGCGGCAGc -3' miRNA: 3'- guaUCGCGUC-----------AUGGC-AGCa--CGUCGUC- -5' |
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12126 | 3' | -54.1 | NC_003309.1 | + | 1252 | 0.68 | 0.596053 |
Target: 5'- --cAGCGCGGcGCUGUCGUGCucGUg- -3' miRNA: 3'- guaUCGCGUCaUGGCAGCACGu-CGuc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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