miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12127 5' -51.1 NC_003309.1 + 16674 1.07 0.003518
Target:  5'- aCGGCGAGACGAUGAACUCUUCCGAAAc -3'
miRNA:   3'- -GCCGCUCUGCUACUUGAGAAGGCUUU- -5'
12127 5' -51.1 NC_003309.1 + 5358 0.69 0.770145
Target:  5'- gGcGCGGGAUGGUGAugUUgccgUUCGAGAg -3'
miRNA:   3'- gC-CGCUCUGCUACUugAGa---AGGCUUU- -5'
12127 5' -51.1 NC_003309.1 + 34155 0.66 0.903546
Target:  5'- cCGcGCGAaacGCGAUGAGCUUUUgcaaCCGGAGa -3'
miRNA:   3'- -GC-CGCUc--UGCUACUUGAGAA----GGCUUU- -5'
12127 5' -51.1 NC_003309.1 + 42650 0.66 0.900731
Target:  5'- cCGGCGAuGGCGuccugucgucguuUGAACgguaUUCCGAAGg -3'
miRNA:   3'- -GCCGCU-CUGCu------------ACUUGag--AAGGCUUU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.