Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12130 | 5' | -54.9 | NC_003309.1 | + | 46338 | 0.67 | 0.702162 |
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Target: 5'- aCG-CGGACGUacgCGUGCCGcCGuacCGCCg -3' miRNA: 3'- -GCaGCUUGCA---GUACGGCuGCuc-GCGG- -5' |
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| 12130 | 5' | -54.9 | NC_003309.1 | + | 10473 | 0.67 | 0.702162 |
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Target: 5'- -aUCGcGCG-CAUGCCG-CGAGUucgGCCu -3' miRNA: 3'- gcAGCuUGCaGUACGGCuGCUCG---CGG- -5' |
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| 12130 | 5' | -54.9 | NC_003309.1 | + | 1299 | 0.67 | 0.702162 |
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Target: 5'- --aUGAGCGgc-UGUCGACGcGCGCCc -3' miRNA: 3'- gcaGCUUGCaguACGGCUGCuCGCGG- -5' |
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| 12130 | 5' | -54.9 | NC_003309.1 | + | 42660 | 0.67 | 0.712795 |
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Target: 5'- aG-CGAACGg---GCCGGCGAugGCGUCc -3' miRNA: 3'- gCaGCUUGCaguaCGGCUGCU--CGCGG- -5' |
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| 12130 | 5' | -54.9 | NC_003309.1 | + | 44317 | 0.67 | 0.717027 |
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Target: 5'- aCGUCGGugACGgcugccgagaacgauUCGuuuugcuucUGCCGcuuugcACGAGCGCCg -3' miRNA: 3'- -GCAGCU--UGC---------------AGU---------ACGGC------UGCUCGCGG- -5' |
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| 12130 | 5' | -54.9 | NC_003309.1 | + | 10652 | 0.66 | 0.784435 |
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Target: 5'- cCG-CGuACGgCAUGCCcGCGAugcgcGCGCCg -3' miRNA: 3'- -GCaGCuUGCaGUACGGcUGCU-----CGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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