Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12132 | 3' | -61.9 | NC_003309.1 | + | 36842 | 0.66 | 0.421247 |
Target: 5'- uCGGAUAUgGGCgGCCuuUaagGGUGGCCa -3' miRNA: 3'- -GCCUAUG-CCGgCGGcuAgg-CCGCCGG- -5' |
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12132 | 3' | -61.9 | NC_003309.1 | + | 10893 | 0.66 | 0.411463 |
Target: 5'- gCGGucGCGGUCGCCGcgcguUCCGcucgcucGCGcGCCu -3' miRNA: 3'- -GCCuaUGCCGGCGGCu----AGGC-------CGC-CGG- -5' |
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12132 | 3' | -61.9 | NC_003309.1 | + | 43538 | 0.66 | 0.386358 |
Target: 5'- ----cACGcGCaCGCCG--UCGGCGGCCa -3' miRNA: 3'- gccuaUGC-CG-GCGGCuaGGCCGCCGG- -5' |
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12132 | 3' | -61.9 | NC_003309.1 | + | 16007 | 0.67 | 0.35343 |
Target: 5'- uCGGAUGCGaGCgCGaauUGGUCCGucggcGCGGCUg -3' miRNA: 3'- -GCCUAUGC-CG-GCg--GCUAGGC-----CGCCGG- -5' |
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12132 | 3' | -61.9 | NC_003309.1 | + | 44534 | 0.67 | 0.336954 |
Target: 5'- cCGGGUugaguucGCaGCCGauGAaaCGGCGGCCg -3' miRNA: 3'- -GCCUA-------UGcCGGCggCUagGCCGCCGG- -5' |
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12132 | 3' | -61.9 | NC_003309.1 | + | 46336 | 0.68 | 0.330067 |
Target: 5'- gCGGAcgUACGcguGCCGCCGuaccgCCGaGCacGGCCu -3' miRNA: 3'- -GCCU--AUGC---CGGCGGCua---GGC-CG--CCGG- -5' |
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12132 | 3' | -61.9 | NC_003309.1 | + | 41292 | 0.68 | 0.322536 |
Target: 5'- gGGAUgggaACGGCU--UGAcgCUGGCGGCCg -3' miRNA: 3'- gCCUA----UGCCGGcgGCUa-GGCCGCCGG- -5' |
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12132 | 3' | -61.9 | NC_003309.1 | + | 9426 | 0.68 | 0.305705 |
Target: 5'- -cGAgcCGGCCGUCGAcgagagaccgcgcaUgCGGCGGUCg -3' miRNA: 3'- gcCUauGCCGGCGGCU--------------AgGCCGCCGG- -5' |
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12132 | 3' | -61.9 | NC_003309.1 | + | 49951 | 0.68 | 0.300718 |
Target: 5'- --------aCCGUCGAUUCGGCGGCCg -3' miRNA: 3'- gccuaugccGGCGGCUAGGCCGCCGG- -5' |
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12132 | 3' | -61.9 | NC_003309.1 | + | 44924 | 0.68 | 0.293703 |
Target: 5'- --cGUGCGGCCGCCucgggcGUCCaGCGGUa -3' miRNA: 3'- gccUAUGCCGGCGGc-----UAGGcCGCCGg -5' |
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12132 | 3' | -61.9 | NC_003309.1 | + | 15328 | 0.68 | 0.293703 |
Target: 5'- uCGGcgAUGGCUGCUgcgcgucgGAUgucugcucgaagCCGGUGGCCu -3' miRNA: 3'- -GCCuaUGCCGGCGG--------CUA------------GGCCGCCGG- -5' |
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12132 | 3' | -61.9 | NC_003309.1 | + | 45271 | 0.69 | 0.268211 |
Target: 5'- gCGGAUuuggucgaaugcCGGCCGCCGcgUgcgccucgaauacguCGGCGGCa -3' miRNA: 3'- -GCCUAu-----------GCCGGCGGCuaG---------------GCCGCCGg -5' |
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12132 | 3' | -61.9 | NC_003309.1 | + | 15019 | 0.69 | 0.260541 |
Target: 5'- aCGGAaggucagcgACGGCCGCCccUUCGaCGGCCc -3' miRNA: 3'- -GCCUa--------UGCCGGCGGcuAGGCcGCCGG- -5' |
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12132 | 3' | -61.9 | NC_003309.1 | + | 31511 | 0.69 | 0.248156 |
Target: 5'- -----cCGGCCaGCCGA--CGGCGGCCu -3' miRNA: 3'- gccuauGCCGG-CGGCUagGCCGCCGG- -5' |
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12132 | 3' | -61.9 | NC_003309.1 | + | 8546 | 0.7 | 0.236261 |
Target: 5'- gGGAguUAC-GCCGCCGG--CGGCGGCa -3' miRNA: 3'- gCCU--AUGcCGGCGGCUagGCCGCCGg -5' |
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12132 | 3' | -61.9 | NC_003309.1 | + | 8712 | 0.7 | 0.236261 |
Target: 5'- --cAUGCGGUCGCCGA-CCGuCGGCUc -3' miRNA: 3'- gccUAUGCCGGCGGCUaGGCcGCCGG- -5' |
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12132 | 3' | -61.9 | NC_003309.1 | + | 22492 | 0.7 | 0.224848 |
Target: 5'- uGGucgacgGCGGCCaccGCCGAcgCGGCGGCa -3' miRNA: 3'- gCCua----UGCCGG---CGGCUagGCCGCCGg -5' |
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12132 | 3' | -61.9 | NC_003309.1 | + | 22665 | 0.71 | 0.198354 |
Target: 5'- aGGcgGCGGCaccauCGCCcacgCCGGCGGCg -3' miRNA: 3'- gCCuaUGCCG-----GCGGcua-GGCCGCCGg -5' |
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12132 | 3' | -61.9 | NC_003309.1 | + | 45864 | 0.72 | 0.157493 |
Target: 5'- gCGGcgACGGCCGUCGAUCacgaGGUucucGCCc -3' miRNA: 3'- -GCCuaUGCCGGCGGCUAGg---CCGc---CGG- -5' |
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12132 | 3' | -61.9 | NC_003309.1 | + | 7835 | 0.74 | 0.127725 |
Target: 5'- cCGGA-ACGGCCGCCaGUcagcCCGGCGagcGCCa -3' miRNA: 3'- -GCCUaUGCCGGCGGcUA----GGCCGC---CGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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