Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12132 | 3' | -61.9 | NC_003309.1 | + | 49951 | 0.68 | 0.300718 |
Target: 5'- --------aCCGUCGAUUCGGCGGCCg -3' miRNA: 3'- gccuaugccGGCGGCUAGGCCGCCGG- -5' |
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12132 | 3' | -61.9 | NC_003309.1 | + | 44924 | 0.68 | 0.293703 |
Target: 5'- --cGUGCGGCCGCCucgggcGUCCaGCGGUa -3' miRNA: 3'- gccUAUGCCGGCGGc-----UAGGcCGCCGg -5' |
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12132 | 3' | -61.9 | NC_003309.1 | + | 15328 | 0.68 | 0.293703 |
Target: 5'- uCGGcgAUGGCUGCUgcgcgucgGAUgucugcucgaagCCGGUGGCCu -3' miRNA: 3'- -GCCuaUGCCGGCGG--------CUA------------GGCCGCCGG- -5' |
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12132 | 3' | -61.9 | NC_003309.1 | + | 18700 | 1.11 | 0.000187 |
Target: 5'- gCGGAUACGGCCGCCGAUCCGGCGGCCg -3' miRNA: 3'- -GCCUAUGCCGGCGGCUAGGCCGCCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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