Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12133 | 3' | -47.8 | NC_003309.1 | + | 165 | 0.66 | 0.98554 |
Target: 5'- cCGCGAUuuuccgCGCCGUCGGCuuggGUUUUCu--- -3' miRNA: 3'- -GCGCUA------GUGGUAGCUG----CAAAAGcuuu -5' |
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12133 | 3' | -47.8 | NC_003309.1 | + | 14348 | 0.66 | 0.983506 |
Target: 5'- aGCGAcuacuUCGCCGUUGACG----CGAAAc -3' miRNA: 3'- gCGCU-----AGUGGUAGCUGCaaaaGCUUU- -5' |
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12133 | 3' | -47.8 | NC_003309.1 | + | 35722 | 0.66 | 0.983506 |
Target: 5'- aCGCGaAUCGCUcuUCGACGgcUUCGu-- -3' miRNA: 3'- -GCGC-UAGUGGu-AGCUGCaaAAGCuuu -5' |
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12133 | 3' | -47.8 | NC_003309.1 | + | 51566 | 0.66 | 0.978797 |
Target: 5'- cCGCGAUUGCC-UCGGCaac-UCGAAGc -3' miRNA: 3'- -GCGCUAGUGGuAGCUGcaaaAGCUUU- -5' |
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12133 | 3' | -47.8 | NC_003309.1 | + | 9513 | 0.67 | 0.972228 |
Target: 5'- gCGCGcgCACCGUgaccugcacgcuugCGGCGU--UCGAGg -3' miRNA: 3'- -GCGCuaGUGGUA--------------GCUGCAaaAGCUUu -5' |
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12133 | 3' | -47.8 | NC_003309.1 | + | 34533 | 0.68 | 0.945008 |
Target: 5'- aGCGAgCAcCCGUCGAUGUgggcggccgUCGAAu -3' miRNA: 3'- gCGCUaGU-GGUAGCUGCAaa-------AGCUUu -5' |
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12133 | 3' | -47.8 | NC_003309.1 | + | 23050 | 0.68 | 0.945008 |
Target: 5'- cCGCGAaCACC-UCGACGUaacagucgugcUUCGGAAa -3' miRNA: 3'- -GCGCUaGUGGuAGCUGCAa----------AAGCUUU- -5' |
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12133 | 3' | -47.8 | NC_003309.1 | + | 11970 | 0.68 | 0.939822 |
Target: 5'- gGCGcgCGCCgcGUCGGCGUUgaagcCGAAc -3' miRNA: 3'- gCGCuaGUGG--UAGCUGCAAaa---GCUUu -5' |
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12133 | 3' | -47.8 | NC_003309.1 | + | 14802 | 0.69 | 0.928531 |
Target: 5'- cCGCGcagccgCGCCAUU-ACGUUUUCGAGu -3' miRNA: 3'- -GCGCua----GUGGUAGcUGCAAAAGCUUu -5' |
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12133 | 3' | -47.8 | NC_003309.1 | + | 18485 | 0.69 | 0.922424 |
Target: 5'- gCGCGAUC-CCGUCGucguccugcACGgcUUCGAc- -3' miRNA: 3'- -GCGCUAGuGGUAGC---------UGCaaAAGCUuu -5' |
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12133 | 3' | -47.8 | NC_003309.1 | + | 50058 | 0.7 | 0.902267 |
Target: 5'- gCGCGAUCACCGcccaucgcUCGACGUccaUCa--- -3' miRNA: 3'- -GCGCUAGUGGU--------AGCUGCAaa-AGcuuu -5' |
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12133 | 3' | -47.8 | NC_003309.1 | + | 35030 | 0.7 | 0.887323 |
Target: 5'- uGCGA-CGCCGaCGACGUgaUCGAu- -3' miRNA: 3'- gCGCUaGUGGUaGCUGCAaaAGCUuu -5' |
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12133 | 3' | -47.8 | NC_003309.1 | + | 28679 | 0.7 | 0.879413 |
Target: 5'- uGgGAUCGCCcUCGGCGUUgaCGGu- -3' miRNA: 3'- gCgCUAGUGGuAGCUGCAAaaGCUuu -5' |
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12133 | 3' | -47.8 | NC_003309.1 | + | 37031 | 0.73 | 0.77571 |
Target: 5'- uCGCGAUCGCCAagGGCGaggcCGAAGc -3' miRNA: 3'- -GCGCUAGUGGUagCUGCaaaaGCUUU- -5' |
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12133 | 3' | -47.8 | NC_003309.1 | + | 19142 | 1.08 | 0.00741 |
Target: 5'- aCGCGAUCACCAUCGACGUUUUCGAAAu -3' miRNA: 3'- -GCGCUAGUGGUAGCUGCAAAAGCUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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