Results 1 - 20 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12134 | 3' | -58.5 | NC_003309.1 | + | 9422 | 0.66 | 0.59185 |
Target: 5'- cCGGcCGuCGACGagaGACCGcgcauGCGGCGGu -3' miRNA: 3'- cGCCaGUcGCUGUg--CUGGC-----UGCCGCC- -5' |
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12134 | 3' | -58.5 | NC_003309.1 | + | 10044 | 0.66 | 0.59185 |
Target: 5'- cGCGcGUCAGCGcCucgACGAgauuaCCGACGGUc- -3' miRNA: 3'- -CGC-CAGUCGCuG---UGCU-----GGCUGCCGcc -5' |
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12134 | 3' | -58.5 | NC_003309.1 | + | 43563 | 0.66 | 0.575107 |
Target: 5'- aCGG-CGGCGacgugacgaucgucuGCACGcgcacGCCGuCGGCGGc -3' miRNA: 3'- cGCCaGUCGC---------------UGUGC-----UGGCuGCCGCC- -5' |
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12134 | 3' | -58.5 | NC_003309.1 | + | 37947 | 0.66 | 0.570937 |
Target: 5'- cGCGGgauuuccucUCGGauaaGCGCGGCCuuuCGGCGGg -3' miRNA: 3'- -CGCC---------AGUCgc--UGUGCUGGcu-GCCGCC- -5' |
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12134 | 3' | -58.5 | NC_003309.1 | + | 28197 | 0.66 | 0.570937 |
Target: 5'- --cGUCAGgGGCGCGAUaguuggugcacaCGACGGCa- -3' miRNA: 3'- cgcCAGUCgCUGUGCUG------------GCUGCCGcc -5' |
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12134 | 3' | -58.5 | NC_003309.1 | + | 4618 | 0.66 | 0.602356 |
Target: 5'- uCGG-CAGCGAUGCGAUUGAuugcugccugUGGCGu -3' miRNA: 3'- cGCCaGUCGCUGUGCUGGCU----------GCCGCc -5' |
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12134 | 3' | -58.5 | NC_003309.1 | + | 25761 | 0.67 | 0.519576 |
Target: 5'- cGCGG--AGCG-CAUGAUCGGCGaCGGa -3' miRNA: 3'- -CGCCagUCGCuGUGCUGGCUGCcGCC- -5' |
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12134 | 3' | -58.5 | NC_003309.1 | + | 31537 | 0.67 | 0.519576 |
Target: 5'- gGCGGcaAGCGccagcucgaacuACGCcggccaGCCGACGGCGGc -3' miRNA: 3'- -CGCCagUCGC------------UGUGc-----UGGCUGCCGCC- -5' |
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12134 | 3' | -58.5 | NC_003309.1 | + | 31476 | 0.67 | 0.53993 |
Target: 5'- cCGG-CGGCGAaaGCGGCC-ACGGCGc -3' miRNA: 3'- cGCCaGUCGCUg-UGCUGGcUGCCGCc -5' |
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12134 | 3' | -58.5 | NC_003309.1 | + | 13114 | 0.67 | 0.560546 |
Target: 5'- -aGGUguGCGcGCACGcCCGGCuGCGa -3' miRNA: 3'- cgCCAguCGC-UGUGCuGGCUGcCGCc -5' |
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12134 | 3' | -58.5 | NC_003309.1 | + | 20042 | 0.68 | 0.479836 |
Target: 5'- -aGGUCGGCG-UACGACuucgCGACGGUu- -3' miRNA: 3'- cgCCAGUCGCuGUGCUG----GCUGCCGcc -5' |
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12134 | 3' | -58.5 | NC_003309.1 | + | 21643 | 0.68 | 0.470132 |
Target: 5'- uCGGUCAacguaagcGaCGACGCGACCGACGcuaugcuucccGCGu -3' miRNA: 3'- cGCCAGU--------C-GCUGUGCUGGCUGC-----------CGCc -5' |
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12134 | 3' | -58.5 | NC_003309.1 | + | 14577 | 0.68 | 0.451031 |
Target: 5'- -aGGUaCAGCGACGCuugcguauGAagcccuguugcaCCGACGGCGa -3' miRNA: 3'- cgCCA-GUCGCUGUG--------CU------------GGCUGCCGCc -5' |
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12134 | 3' | -58.5 | NC_003309.1 | + | 26951 | 0.69 | 0.432363 |
Target: 5'- --cGUCAGCG-CGCGaacaccGCCGACGGUGu -3' miRNA: 3'- cgcCAGUCGCuGUGC------UGGCUGCCGCc -5' |
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12134 | 3' | -58.5 | NC_003309.1 | + | 15014 | 0.69 | 0.396424 |
Target: 5'- -aGGUCAGCGACGgcCGccccuUCGACGGCc- -3' miRNA: 3'- cgCCAGUCGCUGU--GCu----GGCUGCCGcc -5' |
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12134 | 3' | -58.5 | NC_003309.1 | + | 22665 | 0.69 | 0.405228 |
Target: 5'- -aGG-CGGCGGCACcaucgcccacGCCGGCGGCGc -3' miRNA: 3'- cgCCaGUCGCUGUGc---------UGGCUGCCGCc -5' |
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12134 | 3' | -58.5 | NC_003309.1 | + | 47491 | 0.69 | 0.405228 |
Target: 5'- gGCGGcgCAGCGACACcuucGCCGcCgGGCGu -3' miRNA: 3'- -CGCCa-GUCGCUGUGc---UGGCuG-CCGCc -5' |
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12134 | 3' | -58.5 | NC_003309.1 | + | 22589 | 0.69 | 0.405228 |
Target: 5'- uCGGaCAGCGACGCGcaGCCGcucagugccaGCGGCa- -3' miRNA: 3'- cGCCaGUCGCUGUGC--UGGC----------UGCCGcc -5' |
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12134 | 3' | -58.5 | NC_003309.1 | + | 11982 | 0.69 | 0.414154 |
Target: 5'- cGCGG-CAgccGCGGCGCGcGCCGcguCGGCGu -3' miRNA: 3'- -CGCCaGU---CGCUGUGC-UGGCu--GCCGCc -5' |
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12134 | 3' | -58.5 | NC_003309.1 | + | 18711 | 0.69 | 0.4232 |
Target: 5'- cCGG-CuGCGGCGCGgauacggccGCCGAucCGGCGGc -3' miRNA: 3'- cGCCaGuCGCUGUGC---------UGGCU--GCCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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