Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12135 | 5' | -53.9 | NC_003309.1 | + | 12063 | 0.71 | 0.477674 |
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Target: 5'- gGCCGCCGCGgcGUUGguggauaCGCgugacgucUGCGCc -3' miRNA: 3'- -CGGCGGCGCaaCAGCaa-----GUGa-------ACGCG- -5' |
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| 12135 | 5' | -53.9 | NC_003309.1 | + | 12131 | 0.72 | 0.447609 |
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Target: 5'- cGCUGCCGgaUGUUGUCGa--GCaUGCGCu -3' miRNA: 3'- -CGGCGGC--GCAACAGCaagUGaACGCG- -5' |
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| 12135 | 5' | -53.9 | NC_003309.1 | + | 22391 | 0.73 | 0.381808 |
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Target: 5'- uGCCGCUGCGc-GUCGaaCGCccGCGCg -3' miRNA: 3'- -CGGCGGCGCaaCAGCaaGUGaaCGCG- -5' |
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| 12135 | 5' | -53.9 | NC_003309.1 | + | 45252 | 0.77 | 0.241753 |
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Target: 5'- gGCCGCCGCGUgcgccucgaauacGUCGgcggCACUccaGCGCa -3' miRNA: 3'- -CGGCGGCGCAa------------CAGCaa--GUGAa--CGCG- -5' |
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| 12135 | 5' | -53.9 | NC_003309.1 | + | 9722 | 0.78 | 0.194529 |
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Target: 5'- gGCCGUCGCGUcgagcgguugccgugUGUCGcgCGCgaUGCGCa -3' miRNA: 3'- -CGGCGGCGCA---------------ACAGCaaGUGa-ACGCG- -5' |
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| 12135 | 5' | -53.9 | NC_003309.1 | + | 20010 | 1.13 | 0.000732 |
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Target: 5'- cGCCGCCGCGUUGUCGUUCACUUGCGCc -3' miRNA: 3'- -CGGCGGCGCAACAGCAAGUGAACGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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