Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12136 | 5' | -62 | NC_003309.1 | + | 13450 | 0.66 | 0.345453 |
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Target: 5'- aCGCaGCGUcguaucGCCG-CCGUGCCCUUg- -3' miRNA: 3'- -GCGgCGCGa-----CGGCaGGUACGGGAGaa -5' |
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| 12136 | 5' | -62 | NC_003309.1 | + | 10451 | 0.66 | 0.337448 |
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Target: 5'- gGCCuGCGCUGCCGccUCCGccGCCUgcgCg- -3' miRNA: 3'- gCGG-CGCGACGGC--AGGUa-CGGGa--Gaa -5' |
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| 12136 | 5' | -62 | NC_003309.1 | + | 5012 | 0.68 | 0.239587 |
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Target: 5'- aGCCGcCGCaGCCG-CCAUGCgUUCa- -3' miRNA: 3'- gCGGC-GCGaCGGCaGGUACGgGAGaa -5' |
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| 12136 | 5' | -62 | NC_003309.1 | + | 10928 | 0.7 | 0.194565 |
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Target: 5'- uCGCCGCGCUuucagucGCCGUCUucaGCCC-Cg- -3' miRNA: 3'- -GCGGCGCGA-------CGGCAGGua-CGGGaGaa -5' |
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| 12136 | 5' | -62 | NC_003309.1 | + | 20008 | 0.73 | 0.117353 |
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Target: 5'- cCGCCGCGUUGUCGUUCAcuUGCgCCUgUa -3' miRNA: 3'- -GCGGCGCGACGGCAGGU--ACG-GGAgAa -5' |
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| 12136 | 5' | -62 | NC_003309.1 | + | 27144 | 0.75 | 0.071261 |
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Target: 5'- uGCCGCGCUGaagaaUGUCCAUGCUUUCc- -3' miRNA: 3'- gCGGCGCGACg----GCAGGUACGGGAGaa -5' |
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| 12136 | 5' | -62 | NC_003309.1 | + | 20603 | 1.05 | 0.000329 |
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Target: 5'- cCGCCGCGCUGCCGUCCAUGCCCUCUUg -3' miRNA: 3'- -GCGGCGCGACGGCAGGUACGGGAGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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