Results 1 - 20 of 34 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12137 | 3' | -51.3 | NC_003309.1 | + | 18381 | 0.66 | 0.924195 |
Target: 5'- uGCCGacguacuUGAauGAAccGCCGACGAcguUCGCgGCCg -3' miRNA: 3'- -CGGC-------ACU--CUUa-UGGCUGCU---AGUG-CGG- -5' |
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12137 | 3' | -51.3 | NC_003309.1 | + | 1001 | 0.66 | 0.918744 |
Target: 5'- cGUCGUcGAGGAcgaacaugccgaUGCCGACGccCGCGgCg -3' miRNA: 3'- -CGGCA-CUCUU------------AUGGCUGCuaGUGCgG- -5' |
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12137 | 3' | -51.3 | NC_003309.1 | + | 48036 | 0.66 | 0.918744 |
Target: 5'- cGgCGUGaAGAGUgGCCGGuCGAgguCGCCg -3' miRNA: 3'- -CgGCAC-UCUUA-UGGCU-GCUaguGCGG- -5' |
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12137 | 3' | -51.3 | NC_003309.1 | + | 31727 | 0.66 | 0.912419 |
Target: 5'- -gCGUGAGug-GCCGugGccgcauGUCugGCUc -3' miRNA: 3'- cgGCACUCuuaUGGCugC------UAGugCGG- -5' |
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12137 | 3' | -51.3 | NC_003309.1 | + | 12849 | 0.67 | 0.898928 |
Target: 5'- aCCGcgcGAGG---UCGGCGAUCAUGCUg -3' miRNA: 3'- cGGCa--CUCUuauGGCUGCUAGUGCGG- -5' |
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12137 | 3' | -51.3 | NC_003309.1 | + | 15237 | 0.67 | 0.898928 |
Target: 5'- cGUCGacUGAaGAUGCCGAUGAaaGCGCa -3' miRNA: 3'- -CGGC--ACUcUUAUGGCUGCUagUGCGg -5' |
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12137 | 3' | -51.3 | NC_003309.1 | + | 17903 | 0.67 | 0.898928 |
Target: 5'- -aCGUGcgucGAacgucAUGCCGACGA--GCGCCg -3' miRNA: 3'- cgGCACu---CU-----UAUGGCUGCUagUGCGG- -5' |
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12137 | 3' | -51.3 | NC_003309.1 | + | 14192 | 0.67 | 0.891768 |
Target: 5'- cGCCGcgaaGAGAucgcUACCcACGGUCAgcuUGCCg -3' miRNA: 3'- -CGGCa---CUCUu---AUGGcUGCUAGU---GCGG- -5' |
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12137 | 3' | -51.3 | NC_003309.1 | + | 24443 | 0.67 | 0.868687 |
Target: 5'- cCCGUGAcgcggcGGAUAUCGGCGcUCAugagcaaaaccCGCCg -3' miRNA: 3'- cGGCACU------CUUAUGGCUGCuAGU-----------GCGG- -5' |
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12137 | 3' | -51.3 | NC_003309.1 | + | 16077 | 0.67 | 0.867877 |
Target: 5'- uGCCGacgcccccggaUGcGAAUGCacgagagCGACGAUCuCGCCa -3' miRNA: 3'- -CGGC-----------ACuCUUAUG-------GCUGCUAGuGCGG- -5' |
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12137 | 3' | -51.3 | NC_003309.1 | + | 15840 | 0.68 | 0.860479 |
Target: 5'- cGCCGUGuGGcggguccucAUAUCGAaacuguuucgUGcAUCACGCCa -3' miRNA: 3'- -CGGCACuCU---------UAUGGCU----------GC-UAGUGCGG- -5' |
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12137 | 3' | -51.3 | NC_003309.1 | + | 18976 | 0.68 | 0.860479 |
Target: 5'- uGUCGcguUGAucGUGuaGACGAUCugGCCg -3' miRNA: 3'- -CGGC---ACUcuUAUggCUGCUAGugCGG- -5' |
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12137 | 3' | -51.3 | NC_003309.1 | + | 44439 | 0.68 | 0.860479 |
Target: 5'- cCCGgccGAcUGCCGGCGAgCACGCa -3' miRNA: 3'- cGGCacuCUuAUGGCUGCUaGUGCGg -5' |
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12137 | 3' | -51.3 | NC_003309.1 | + | 34971 | 0.68 | 0.860479 |
Target: 5'- aGCUGcUGAuGGAUAuCCaGAC-AUCGCGCCg -3' miRNA: 3'- -CGGC-ACU-CUUAU-GG-CUGcUAGUGCGG- -5' |
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12137 | 3' | -51.3 | NC_003309.1 | + | 15470 | 0.68 | 0.843338 |
Target: 5'- cGCCGUcGGAuUGCCGGcCGGaaguUCACGgCg -3' miRNA: 3'- -CGGCAcUCUuAUGGCU-GCU----AGUGCgG- -5' |
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12137 | 3' | -51.3 | NC_003309.1 | + | 16766 | 0.68 | 0.843338 |
Target: 5'- -aCGUGAGGccGCCGuCG--CGCGCCg -3' miRNA: 3'- cgGCACUCUuaUGGCuGCuaGUGCGG- -5' |
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12137 | 3' | -51.3 | NC_003309.1 | + | 16947 | 0.69 | 0.806401 |
Target: 5'- gGCUGcGcGAA-ACCGaacgucGCGAUCGCGCCg -3' miRNA: 3'- -CGGCaCuCUUaUGGC------UGCUAGUGCGG- -5' |
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12137 | 3' | -51.3 | NC_003309.1 | + | 40159 | 0.69 | 0.800585 |
Target: 5'- aGCCGUGAGAcgugggcgGUACucggccuaucucauCGGuacagcgagaacauCGGUCGCGCCc -3' miRNA: 3'- -CGGCACUCU--------UAUG--------------GCU--------------GCUAGUGCGG- -5' |
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12137 | 3' | -51.3 | NC_003309.1 | + | 18517 | 0.69 | 0.796672 |
Target: 5'- cGCCGUGAccu--CCGucuUGAUgACGCCg -3' miRNA: 3'- -CGGCACUcuuauGGCu--GCUAgUGCGG- -5' |
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12137 | 3' | -51.3 | NC_003309.1 | + | 1196 | 0.69 | 0.796672 |
Target: 5'- cGCCGUcgccuGGGu-UGCCGAUGAUCGgcucaaaucggcUGCCg -3' miRNA: 3'- -CGGCA-----CUCuuAUGGCUGCUAGU------------GCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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