Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12139 | 5' | -61.3 | NC_003309.1 | + | 43290 | 0.66 | 0.395334 |
Target: 5'- uCAGUUCGACgaUGGCcGCGUCGCAgcacucaacggcgcUCGg -3' miRNA: 3'- -GUCGGGCUG--ACCGaCGCGGCGU--------------AGCa -5' |
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12139 | 5' | -61.3 | NC_003309.1 | + | 18453 | 0.66 | 0.38048 |
Target: 5'- aCAGCCUGcuguACUGGUU-CGUCGgAUCGUu -3' miRNA: 3'- -GUCGGGC----UGACCGAcGCGGCgUAGCA- -5' |
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12139 | 5' | -61.3 | NC_003309.1 | + | 33854 | 0.66 | 0.371919 |
Target: 5'- uGGCaaGGCUGGCUGUacGUgGCAUuCGUg -3' miRNA: 3'- gUCGggCUGACCGACG--CGgCGUA-GCA- -5' |
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12139 | 5' | -61.3 | NC_003309.1 | + | 15334 | 0.66 | 0.355193 |
Target: 5'- gCAGCgUCGGCgaUGGCUGCuGC-GCGUCGg -3' miRNA: 3'- -GUCG-GGCUG--ACCGACG-CGgCGUAGCa -5' |
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12139 | 5' | -61.3 | NC_003309.1 | + | 6698 | 0.67 | 0.343805 |
Target: 5'- uCGGCCCaACUGGacaagcucgaaaGCGCCuGCGUCGa -3' miRNA: 3'- -GUCGGGcUGACCga----------CGCGG-CGUAGCa -5' |
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12139 | 5' | -61.3 | NC_003309.1 | + | 18992 | 0.67 | 0.323359 |
Target: 5'- -uGCCCGGCUcauaCUGUGUCGCGUUGa -3' miRNA: 3'- guCGGGCUGAcc--GACGCGGCGUAGCa -5' |
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12139 | 5' | -61.3 | NC_003309.1 | + | 52714 | 0.67 | 0.31574 |
Target: 5'- cCAGCCCGGCuaccggcuccgaguUGGCgauuggcguguguUGUacgaaaucgaagaugGCCGCGUCGUa -3' miRNA: 3'- -GUCGGGCUG--------------ACCG-------------ACG---------------CGGCGUAGCA- -5' |
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12139 | 5' | -61.3 | NC_003309.1 | + | 26486 | 0.69 | 0.234816 |
Target: 5'- aGGCuuGGCUcgcgcgaacGGCUGCGCCGCcuuccCGUu -3' miRNA: 3'- gUCGggCUGA---------CCGACGCGGCGua---GCA- -5' |
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12139 | 5' | -61.3 | NC_003309.1 | + | 19895 | 0.69 | 0.228923 |
Target: 5'- gAGCUCGAUcgcuGCUGCGCCGCGacCGUg -3' miRNA: 3'- gUCGGGCUGac--CGACGCGGCGUa-GCA- -5' |
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12139 | 5' | -61.3 | NC_003309.1 | + | 3577 | 0.7 | 0.219755 |
Target: 5'- cCAGCCCcACUGGCgcccgaccgcguagGCGgCGUAUCGc -3' miRNA: 3'- -GUCGGGcUGACCGa-------------CGCgGCGUAGCa -5' |
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12139 | 5' | -61.3 | NC_003309.1 | + | 10399 | 0.7 | 0.196148 |
Target: 5'- gCAGCUCGAUcgcccugcgcuuUGGCgagcgccgccUGCGCCGCcUCGUg -3' miRNA: 3'- -GUCGGGCUG------------ACCG----------ACGCGGCGuAGCA- -5' |
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12139 | 5' | -61.3 | NC_003309.1 | + | 11978 | 0.71 | 0.17204 |
Target: 5'- gCAGCCgCGGC--GC-GCGCCGCGUCGg -3' miRNA: 3'- -GUCGG-GCUGacCGaCGCGGCGUAGCa -5' |
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12139 | 5' | -61.3 | NC_003309.1 | + | 33658 | 0.74 | 0.10015 |
Target: 5'- aCAGCgaaCGGCUGGCUGagGCCgGCAUCGa -3' miRNA: 3'- -GUCGg--GCUGACCGACg-CGG-CGUAGCa -5' |
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12139 | 5' | -61.3 | NC_003309.1 | + | 23730 | 1.06 | 0.000363 |
Target: 5'- aCAGCCCGACUGGCUGCGCCGCAUCGUc -3' miRNA: 3'- -GUCGGGCUGACCGACGCGGCGUAGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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