Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12140 | 3' | -55.8 | NC_003309.1 | + | 10717 | 0.66 | 0.70898 |
Target: 5'- cGCgaGCGaagcCUCGGCGCGcucgaGCUCCugCu -3' miRNA: 3'- -CGaaCGCac--GAGCUGUGC-----CGAGGugG- -5' |
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12140 | 3' | -55.8 | NC_003309.1 | + | 23686 | 0.66 | 0.687479 |
Target: 5'- aGCUcGCGgcGCUCGACGCccuucCCGCCg -3' miRNA: 3'- -CGAaCGCa-CGAGCUGUGccga-GGUGG- -5' |
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12140 | 3' | -55.8 | NC_003309.1 | + | 26345 | 0.66 | 0.687479 |
Target: 5'- aGCgacGCGcGCaCGACgACGGCcgcuccgauUCCACCg -3' miRNA: 3'- -CGaa-CGCaCGaGCUG-UGCCG---------AGGUGG- -5' |
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12140 | 3' | -55.8 | NC_003309.1 | + | 22342 | 0.66 | 0.665769 |
Target: 5'- --gUGCGccgaucaggUGCUCGACGCaaGCgccggCCGCCa -3' miRNA: 3'- cgaACGC---------ACGAGCUGUGc-CGa----GGUGG- -5' |
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12140 | 3' | -55.8 | NC_003309.1 | + | 45021 | 0.67 | 0.648306 |
Target: 5'- --cUGCGUGCagaacugccgcuugCGugAUGGaaaUCCGCCg -3' miRNA: 3'- cgaACGCACGa-------------GCugUGCCg--AGGUGG- -5' |
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12140 | 3' | -55.8 | NC_003309.1 | + | 31890 | 0.68 | 0.567669 |
Target: 5'- cGCUUGCGgacgUGUUCGACaACGcGgUCUAUCc -3' miRNA: 3'- -CGAACGC----ACGAGCUG-UGC-CgAGGUGG- -5' |
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12140 | 3' | -55.8 | NC_003309.1 | + | 49100 | 0.68 | 0.556914 |
Target: 5'- uGCUUcgGCGUGC-CGACcCGGUucugaucgaUCCACg -3' miRNA: 3'- -CGAA--CGCACGaGCUGuGCCG---------AGGUGg -5' |
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12140 | 3' | -55.8 | NC_003309.1 | + | 39606 | 0.69 | 0.520834 |
Target: 5'- uGCgaGCGUGCugcgcuUCGugACGGauucaagaucgaaUCCGCCu -3' miRNA: 3'- -CGaaCGCACG------AGCugUGCCg------------AGGUGG- -5' |
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12140 | 3' | -55.8 | NC_003309.1 | + | 11523 | 0.69 | 0.514558 |
Target: 5'- uGCUUGCGcGCccuucugCGACGCgaGGCU-CGCCg -3' miRNA: 3'- -CGAACGCaCGa------GCUGUG--CCGAgGUGG- -5' |
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12140 | 3' | -55.8 | NC_003309.1 | + | 13815 | 0.69 | 0.513515 |
Target: 5'- uCUUgGUGUGUgCGACGCGGUUguugucgCCGCCg -3' miRNA: 3'- cGAA-CGCACGaGCUGUGCCGA-------GGUGG- -5' |
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12140 | 3' | -55.8 | NC_003309.1 | + | 52949 | 0.69 | 0.504169 |
Target: 5'- -----gGUGCUUG-CACGGCUUCAUCg -3' miRNA: 3'- cgaacgCACGAGCuGUGCCGAGGUGG- -5' |
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12140 | 3' | -55.8 | NC_003309.1 | + | 10595 | 0.69 | 0.493871 |
Target: 5'- ---cGCGUGCcgucgCGACAUGcGCUugcgCCGCCg -3' miRNA: 3'- cgaaCGCACGa----GCUGUGC-CGA----GGUGG- -5' |
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12140 | 3' | -55.8 | NC_003309.1 | + | 22019 | 0.7 | 0.473572 |
Target: 5'- aGCcggGCGUGCacgUCagGAC-CGGCUCCGCg -3' miRNA: 3'- -CGaa-CGCACG---AG--CUGuGCCGAGGUGg -5' |
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12140 | 3' | -55.8 | NC_003309.1 | + | 4511 | 0.72 | 0.328802 |
Target: 5'- cGCUUGCGUGCUCaaacCACGGaagUUCGCg -3' miRNA: 3'- -CGAACGCACGAGcu--GUGCCg--AGGUGg -5' |
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12140 | 3' | -55.8 | NC_003309.1 | + | 24041 | 1.14 | 0.00039 |
Target: 5'- uGCUUGCGUGCUCGACACGGCUCCACCg -3' miRNA: 3'- -CGAACGCACGAGCUGUGCCGAGGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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