Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12142 | 5' | -61.7 | NC_003309.1 | + | 3120 | 0.66 | 0.381809 |
Target: 5'- gCUCGAUCacaCCCGaCAgUGCCgugCCGUUCa -3' miRNA: 3'- -GAGCUAGg--GGGUcGUgACGG---GGCGAG- -5' |
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12142 | 5' | -61.7 | NC_003309.1 | + | 21430 | 0.66 | 0.381809 |
Target: 5'- -gCG-UCCCCCaAGaUACguUGCCgCCGCUCa -3' miRNA: 3'- gaGCuAGGGGG-UC-GUG--ACGG-GGCGAG- -5' |
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12142 | 5' | -61.7 | NC_003309.1 | + | 10396 | 0.66 | 0.364012 |
Target: 5'- gCUCGAUCgCCCuGCGCUuuggcgaGCgCCGCc- -3' miRNA: 3'- -GAGCUAGgGGGuCGUGA-------CGgGGCGag -5' |
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12142 | 5' | -61.7 | NC_003309.1 | + | 20627 | 0.66 | 0.356563 |
Target: 5'- -aCGGUUCCCCGcGCGagagauCUGCCgCCGCg- -3' miRNA: 3'- gaGCUAGGGGGU-CGU------GACGG-GGCGag -5' |
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12142 | 5' | -61.7 | NC_003309.1 | + | 36932 | 0.66 | 0.349221 |
Target: 5'- -aCGGcaagcUCCCCCAGUACaugggcucgaacagcGUCCCGUUCg -3' miRNA: 3'- gaGCU-----AGGGGGUCGUGa--------------CGGGGCGAG- -5' |
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12142 | 5' | -61.7 | NC_003309.1 | + | 38125 | 0.66 | 0.348412 |
Target: 5'- -cCGuAUCCCgCGGCaAUUGCUCCGCg- -3' miRNA: 3'- gaGC-UAGGGgGUCG-UGACGGGGCGag -5' |
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12142 | 5' | -61.7 | NC_003309.1 | + | 9910 | 0.67 | 0.295127 |
Target: 5'- uUCaGAUCCUgaCCGcGCaACUGCCCCGC-Cg -3' miRNA: 3'- gAG-CUAGGG--GGU-CG-UGACGGGGCGaG- -5' |
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12142 | 5' | -61.7 | NC_003309.1 | + | 13017 | 0.67 | 0.295127 |
Target: 5'- -cCGGUCCCCa--CACcGCCCCGCc- -3' miRNA: 3'- gaGCUAGGGGgucGUGaCGGGGCGag -5' |
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12142 | 5' | -61.7 | NC_003309.1 | + | 505 | 0.68 | 0.281115 |
Target: 5'- gUCGAg-CCCCAGCAUUGCgCCGa-- -3' miRNA: 3'- gAGCUagGGGGUCGUGACGgGGCgag -5' |
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12142 | 5' | -61.7 | NC_003309.1 | + | 4357 | 0.68 | 0.274309 |
Target: 5'- cCUUGAUCCCCU---GCUGCucauCCCGCUUc -3' miRNA: 3'- -GAGCUAGGGGGucgUGACG----GGGCGAG- -5' |
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12142 | 5' | -61.7 | NC_003309.1 | + | 4887 | 0.74 | 0.103985 |
Target: 5'- cCUCGAUCUgugCCAGCGCuUGCaCCCGCUg -3' miRNA: 3'- -GAGCUAGGg--GGUCGUG-ACG-GGGCGAg -5' |
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12142 | 5' | -61.7 | NC_003309.1 | + | 24707 | 1.09 | 0.000205 |
Target: 5'- gCUCGAUCCCCCAGCACUGCCCCGCUCg -3' miRNA: 3'- -GAGCUAGGGGGUCGUGACGGGGCGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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