Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12144 | 3' | -60.9 | NC_003309.1 | + | 37294 | 0.66 | 0.443975 |
Target: 5'- cGCCcuGAAUcuCGCCGGCGCGucgauGGCGGu- -3' miRNA: 3'- -CGG--CUUGucGUGGCCGCGC-----UCGCCcu -5' |
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12144 | 3' | -60.9 | NC_003309.1 | + | 31944 | 0.66 | 0.410925 |
Target: 5'- cGCaCGAACAGUucuucgccgcccgcgGCCGGCGCcAGCGc-- -3' miRNA: 3'- -CG-GCUUGUCG---------------UGGCCGCGcUCGCccu -5' |
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12144 | 3' | -60.9 | NC_003309.1 | + | 9736 | 0.67 | 0.381152 |
Target: 5'- cGCUGAuACAGCucgGCCGucGCGuCGAGCGGu- -3' miRNA: 3'- -CGGCU-UGUCG---UGGC--CGC-GCUCGCCcu -5' |
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12144 | 3' | -60.9 | NC_003309.1 | + | 10753 | 0.68 | 0.332237 |
Target: 5'- cGCCGccucaAGCGCCGuccgucgcugcuGCGCGAGCGcGAg -3' miRNA: 3'- -CGGCuug--UCGUGGC------------CGCGCUCGCcCU- -5' |
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12144 | 3' | -60.9 | NC_003309.1 | + | 31505 | 0.68 | 0.327607 |
Target: 5'- aGCCGAcgGCGGCcuacauugggugaucGCCGGCGgCGAaaGCGGc- -3' miRNA: 3'- -CGGCU--UGUCG---------------UGGCCGC-GCU--CGCCcu -5' |
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12144 | 3' | -60.9 | NC_003309.1 | + | 26404 | 0.69 | 0.288093 |
Target: 5'- cGUCGAAacaguCGGCaACCGccGCGCGgacGGCGGGAu -3' miRNA: 3'- -CGGCUU-----GUCG-UGGC--CGCGC---UCGCCCU- -5' |
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12144 | 3' | -60.9 | NC_003309.1 | + | 13981 | 0.69 | 0.274438 |
Target: 5'- uUCG-GCcGCACCGacgaCGCGGGCGGGAg -3' miRNA: 3'- cGGCuUGuCGUGGCc---GCGCUCGCCCU- -5' |
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12144 | 3' | -60.9 | NC_003309.1 | + | 50215 | 0.69 | 0.267808 |
Target: 5'- aGCCGAGCaAGCAgacagUCGaGCGCGAGguucgaaaugcCGGGGa -3' miRNA: 3'- -CGGCUUG-UCGU-----GGC-CGCGCUC-----------GCCCU- -5' |
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12144 | 3' | -60.9 | NC_003309.1 | + | 22475 | 0.7 | 0.248693 |
Target: 5'- cGCCGAcgcgGCGGCAUCGuGCGCG-GCGa-- -3' miRNA: 3'- -CGGCU----UGUCGUGGC-CGCGCuCGCccu -5' |
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12144 | 3' | -60.9 | NC_003309.1 | + | 43423 | 0.71 | 0.198059 |
Target: 5'- aUUGAACAGCACCuGUGCGuGCuGGAa -3' miRNA: 3'- cGGCUUGUCGUGGcCGCGCuCGcCCU- -5' |
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12144 | 3' | -60.9 | NC_003309.1 | + | 25859 | 1.1 | 0.000249 |
Target: 5'- cGCCGAACAGCACCGGCGCGAGCGGGAu -3' miRNA: 3'- -CGGCUUGUCGUGGCCGCGCUCGCCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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