Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12145 | 3' | -61.3 | NC_003309.1 | + | 34525 | 0.66 | 0.444566 |
Target: 5'- cCCGUCGaUGUGGGCGGCc--GUCGAAUc -3' miRNA: 3'- -GGCGGC-GCGCCUGCCGcccUAGCUUA- -5' |
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12145 | 3' | -61.3 | NC_003309.1 | + | 5049 | 0.66 | 0.42614 |
Target: 5'- gUGCCGCGaCGG-CGGUcGGAUuCGggUc -3' miRNA: 3'- gGCGGCGC-GCCuGCCGcCCUA-GCuuA- -5' |
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12145 | 3' | -61.3 | NC_003309.1 | + | 28943 | 0.67 | 0.389831 |
Target: 5'- cCCGCCaccGUGCGGGCuuuuucauuuuugGGUGGGAccaaucUCGAu- -3' miRNA: 3'- -GGCGG---CGCGCCUG-------------CCGCCCU------AGCUua -5' |
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12145 | 3' | -61.3 | NC_003309.1 | + | 37284 | 0.68 | 0.333478 |
Target: 5'- cUCGCCgGCGCGucGAUGGCGG--UCGAGg -3' miRNA: 3'- -GGCGG-CGCGC--CUGCCGCCcuAGCUUa -5' |
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12145 | 3' | -61.3 | NC_003309.1 | + | 22477 | 0.68 | 0.325824 |
Target: 5'- aCCGCCGaCGCGG-CGGCaucgugcgcGGcGAUCGc-- -3' miRNA: 3'- -GGCGGC-GCGCCuGCCG---------CC-CUAGCuua -5' |
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12145 | 3' | -61.3 | NC_003309.1 | + | 53310 | 0.68 | 0.310176 |
Target: 5'- uCCGCCGgGCGGGCaacgacgGGCGGGccaccaccacGUCa--- -3' miRNA: 3'- -GGCGGCgCGCCUG-------CCGCCC----------UAGcuua -5' |
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12145 | 3' | -61.3 | NC_003309.1 | + | 51768 | 0.68 | 0.303647 |
Target: 5'- uCUGCaaGCGCGGAUGGUGGcacGUCGAc- -3' miRNA: 3'- -GGCGg-CGCGCCUGCCGCCc--UAGCUua -5' |
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12145 | 3' | -61.3 | NC_003309.1 | + | 36573 | 0.69 | 0.289519 |
Target: 5'- aCCGCCuucucgaacaucGuCGCGGcCGGCGcGAUCGAGa -3' miRNA: 3'- -GGCGG------------C-GCGCCuGCCGCcCUAGCUUa -5' |
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12145 | 3' | -61.3 | NC_003309.1 | + | 9567 | 0.7 | 0.238156 |
Target: 5'- aCGCCGCuGCGG-CGGCuugugcguauucGcGGAUCGAAc -3' miRNA: 3'- gGCGGCG-CGCCuGCCG------------C-CCUAGCUUa -5' |
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12145 | 3' | -61.3 | NC_003309.1 | + | 26387 | 1.08 | 0.000372 |
Target: 5'- aCCGCCGCGCGGACGGCGGGAUCGAAUg -3' miRNA: 3'- -GGCGGCGCGCCUGCCGCCCUAGCUUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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