Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12145 | 5' | -52.7 | NC_003309.1 | + | 44180 | 0.66 | 0.835351 |
Target: 5'- -------cGCGGCGCGCuGCcgcucggcguacGCGACGAg -3' miRNA: 3'- uuuuaucuCGCUGCGCG-CG------------UGCUGCU- -5' |
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12145 | 5' | -52.7 | NC_003309.1 | + | 1094 | 0.66 | 0.834438 |
Target: 5'- -----uGGGCGAgCGCuugaccaucaacuGCGCugGACGGa -3' miRNA: 3'- uuuuauCUCGCU-GCG-------------CGCGugCUGCU- -5' |
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12145 | 5' | -52.7 | NC_003309.1 | + | 17715 | 0.66 | 0.826118 |
Target: 5'- --cGUAGAGCGACGuCGUcuuGC-CGuCGAa -3' miRNA: 3'- uuuUAUCUCGCUGC-GCG---CGuGCuGCU- -5' |
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12145 | 5' | -52.7 | NC_003309.1 | + | 49537 | 0.66 | 0.826118 |
Target: 5'- uGAAGUAGAGauAgGCGCuCACGACa- -3' miRNA: 3'- -UUUUAUCUCgcUgCGCGcGUGCUGcu -5' |
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12145 | 5' | -52.7 | NC_003309.1 | + | 42259 | 0.67 | 0.776942 |
Target: 5'- ----cAGAGCGAuuucgagugcCGCGgGCAUGGCa- -3' miRNA: 3'- uuuuaUCUCGCU----------GCGCgCGUGCUGcu -5' |
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12145 | 5' | -52.7 | NC_003309.1 | + | 33054 | 0.67 | 0.756084 |
Target: 5'- ---uUAuuGCGACuCGCGCACGAuCGAc -3' miRNA: 3'- uuuuAUcuCGCUGcGCGCGUGCU-GCU- -5' |
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12145 | 5' | -52.7 | NC_003309.1 | + | 11973 | 0.68 | 0.734692 |
Target: 5'- -------cGCGGCGCGCGcCGCGuCGGc -3' miRNA: 3'- uuuuaucuCGCUGCGCGC-GUGCuGCU- -5' |
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12145 | 5' | -52.7 | NC_003309.1 | + | 52405 | 0.68 | 0.723827 |
Target: 5'- -----cGAGCuuGGCGCaaucaucuGCGCAUGACGAa -3' miRNA: 3'- uuuuauCUCG--CUGCG--------CGCGUGCUGCU- -5' |
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12145 | 5' | -52.7 | NC_003309.1 | + | 22472 | 0.68 | 0.701821 |
Target: 5'- -----cGAcGCGGCGgcauCGUGCGCGGCGAu -3' miRNA: 3'- uuuuauCU-CGCUGC----GCGCGUGCUGCU- -5' |
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12145 | 5' | -52.7 | NC_003309.1 | + | 31376 | 0.68 | 0.690705 |
Target: 5'- -cAAUGGGGCGA-GUGgGCACcGCGAa -3' miRNA: 3'- uuUUAUCUCGCUgCGCgCGUGcUGCU- -5' |
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12145 | 5' | -52.7 | NC_003309.1 | + | 11782 | 0.71 | 0.533889 |
Target: 5'- -----cGAGCGACuuguacgcgucgGCGCGCcCGGCGAu -3' miRNA: 3'- uuuuauCUCGCUG------------CGCGCGuGCUGCU- -5' |
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12145 | 5' | -52.7 | NC_003309.1 | + | 23983 | 0.73 | 0.439525 |
Target: 5'- -----cGAGCGgaACGCGC-CGCGACGAu -3' miRNA: 3'- uuuuauCUCGC--UGCGCGcGUGCUGCU- -5' |
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12145 | 5' | -52.7 | NC_003309.1 | + | 7798 | 0.75 | 0.330002 |
Target: 5'- -----cGAGCGcgccauguACGCGCGuCACGACGAa -3' miRNA: 3'- uuuuauCUCGC--------UGCGCGC-GUGCUGCU- -5' |
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12145 | 5' | -52.7 | NC_003309.1 | + | 5332 | 0.76 | 0.298251 |
Target: 5'- ----gAGAGCGgcaGCGUGCGCGCGcCGAg -3' miRNA: 3'- uuuuaUCUCGC---UGCGCGCGUGCuGCU- -5' |
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12145 | 5' | -52.7 | NC_003309.1 | + | 26353 | 1.07 | 0.002442 |
Target: 5'- cAAAAUAGAGCGACGCGCGCACGACGAc -3' miRNA: 3'- -UUUUAUCUCGCUGCGCGCGUGCUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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