Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12148 | 5' | -51.6 | NC_003309.1 | + | 21754 | 0.78 | 0.284792 |
Target: 5'- cGCAGGCGUgCAGCGaGCUcGAUcugcUCUCGAc -3' miRNA: 3'- -CGUCUGCAgGUUGC-CGA-CUA----AGAGCU- -5' |
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12148 | 5' | -51.6 | NC_003309.1 | + | 28016 | 1.12 | 0.001637 |
Target: 5'- uGCAGACGUCCAACGGCUGAUUCUCGAa -3' miRNA: 3'- -CGUCUGCAGGUUGCCGACUAAGAGCU- -5' |
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12148 | 5' | -51.6 | NC_003309.1 | + | 32907 | 0.7 | 0.691002 |
Target: 5'- uGCGGcCGgacugCgCGACGGCagGGUUCUCGGg -3' miRNA: 3'- -CGUCuGCa----G-GUUGCCGa-CUAAGAGCU- -5' |
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12148 | 5' | -51.6 | NC_003309.1 | + | 44912 | 0.72 | 0.601299 |
Target: 5'- uCGGGCGUCCAGCGGUacaUGAa-CUCGc -3' miRNA: 3'- cGUCUGCAGGUUGCCG---ACUaaGAGCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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