miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12148 5' -51.6 NC_003309.1 + 21754 0.78 0.284792
Target:  5'- cGCAGGCGUgCAGCGaGCUcGAUcugcUCUCGAc -3'
miRNA:   3'- -CGUCUGCAgGUUGC-CGA-CUA----AGAGCU- -5'
12148 5' -51.6 NC_003309.1 + 28016 1.12 0.001637
Target:  5'- uGCAGACGUCCAACGGCUGAUUCUCGAa -3'
miRNA:   3'- -CGUCUGCAGGUUGCCGACUAAGAGCU- -5'
12148 5' -51.6 NC_003309.1 + 32907 0.7 0.691002
Target:  5'- uGCGGcCGgacugCgCGACGGCagGGUUCUCGGg -3'
miRNA:   3'- -CGUCuGCa----G-GUUGCCGa-CUAAGAGCU- -5'
12148 5' -51.6 NC_003309.1 + 44912 0.72 0.601299
Target:  5'- uCGGGCGUCCAGCGGUacaUGAa-CUCGc -3'
miRNA:   3'- cGUCUGCAGGUUGCCG---ACUaaGAGCu -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.