Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12149 | 3' | -58.3 | NC_003309.1 | + | 11501 | 0.68 | 0.457103 |
Target: 5'- gCGAGGcuCGCCgugUCGAuCGCGCCgG-CGg -3' miRNA: 3'- -GCUCCuuGCGG---AGCU-GCGCGGgUaGC- -5' |
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12149 | 3' | -58.3 | NC_003309.1 | + | 28333 | 1.09 | 0.00059 |
Target: 5'- gCGAGGAACGCCUCGACGCGCCCAUCGa -3' miRNA: 3'- -GCUCCUUGCGGAGCUGCGCGGGUAGC- -5' |
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12149 | 3' | -58.3 | NC_003309.1 | + | 11783 | 0.77 | 0.129669 |
Target: 5'- uCGAGcGAcuuguACGCgUCGGCGCGCCCggCGa -3' miRNA: 3'- -GCUC-CU-----UGCGgAGCUGCGCGGGuaGC- -5' |
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12149 | 3' | -58.3 | NC_003309.1 | + | 43378 | 0.75 | 0.165039 |
Target: 5'- aCGAccucGGAGUG-CUCGACGCGCCCGUCu -3' miRNA: 3'- -GCU----CCUUGCgGAGCUGCGCGGGUAGc -5' |
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12149 | 3' | -58.3 | NC_003309.1 | + | 10716 | 0.74 | 0.193222 |
Target: 5'- gCGAGcGAA-GCCUCGGCGCGCUCGa-- -3' miRNA: 3'- -GCUC-CUUgCGGAGCUGCGCGGGUagc -5' |
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12149 | 3' | -58.3 | NC_003309.1 | + | 41836 | 0.71 | 0.31121 |
Target: 5'- aGAGGAaagagaucACGCCgacCaACgGCGCCCAUCGu -3' miRNA: 3'- gCUCCU--------UGCGGa--GcUG-CGCGGGUAGC- -5' |
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12149 | 3' | -58.3 | NC_003309.1 | + | 5581 | 0.7 | 0.341329 |
Target: 5'- gCGAauGAACGCCUUGucuucgcGCGCGCCgAUCGc -3' miRNA: 3'- -GCUc-CUUGCGGAGC-------UGCGCGGgUAGC- -5' |
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12149 | 3' | -58.3 | NC_003309.1 | + | 38495 | 0.69 | 0.389028 |
Target: 5'- aGGcGGAACGCCUCGACcguaaagcguGCGCaacgaaagcugcaCGUCGg -3' miRNA: 3'- gCU-CCUUGCGGAGCUG----------CGCGg------------GUAGC- -5' |
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12149 | 3' | -58.3 | NC_003309.1 | + | 51525 | 0.68 | 0.438063 |
Target: 5'- uCGAGGAAaGCCaUCGGCuucgGCGCCCugcCGu -3' miRNA: 3'- -GCUCCUUgCGG-AGCUG----CGCGGGua-GC- -5' |
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12149 | 3' | -58.3 | NC_003309.1 | + | 44409 | 0.68 | 0.446575 |
Target: 5'- uCGAcGAGCGCCUCGGggaaguugcaaagUGgGCgCCGUCGa -3' miRNA: 3'- -GCUcCUUGCGGAGCU-------------GCgCG-GGUAGC- -5' |
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12149 | 3' | -58.3 | NC_003309.1 | + | 52275 | 0.66 | 0.589771 |
Target: 5'- ----cAGCGCaCUUGcCGUGCCCGUCGa -3' miRNA: 3'- gcuccUUGCG-GAGCuGCGCGGGUAGC- -5' |
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12149 | 3' | -58.3 | NC_003309.1 | + | 27757 | 0.68 | 0.466788 |
Target: 5'- -uGGGAGuCGCCagaCGACGCGCCgcccggcgCAUCGc -3' miRNA: 3'- gcUCCUU-GCGGa--GCUGCGCGG--------GUAGC- -5' |
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12149 | 3' | -58.3 | NC_003309.1 | + | 5163 | 0.68 | 0.466788 |
Target: 5'- uCGAGGcgagcuGCGCgUCGccGCGCGCCgCcgCGa -3' miRNA: 3'- -GCUCCu-----UGCGgAGC--UGCGCGG-GuaGC- -5' |
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12149 | 3' | -58.3 | NC_003309.1 | + | 10174 | 0.68 | 0.476578 |
Target: 5'- -------aGCCUCGcgcuCGCGCCCGUCGc -3' miRNA: 3'- gcuccuugCGGAGCu---GCGCGGGUAGC- -5' |
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12149 | 3' | -58.3 | NC_003309.1 | + | 41030 | 0.68 | 0.477562 |
Target: 5'- --cGGGACGCCgccgcugcacaagcgCGACaGCGCCCAa-- -3' miRNA: 3'- gcuCCUUGCGGa--------------GCUG-CGCGGGUagc -5' |
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12149 | 3' | -58.3 | NC_003309.1 | + | 45763 | 0.67 | 0.506535 |
Target: 5'- uCGGGuucGGCGCCUgaUGuACGCGCCgGUCGu -3' miRNA: 3'- -GCUCc--UUGCGGA--GC-UGCGCGGgUAGC- -5' |
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12149 | 3' | -58.3 | NC_003309.1 | + | 22382 | 0.67 | 0.526945 |
Target: 5'- gCGucGAACGCCcgcgCGGCcUGCUCGUCGg -3' miRNA: 3'- -GCucCUUGCGGa---GCUGcGCGGGUAGC- -5' |
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12149 | 3' | -58.3 | NC_003309.1 | + | 9480 | 0.66 | 0.558108 |
Target: 5'- uCGAGGcaAACGCCgcaUCGcugUGCGCCCgccGUCGc -3' miRNA: 3'- -GCUCC--UUGCGG---AGCu--GCGCGGG---UAGC- -5' |
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12149 | 3' | -58.3 | NC_003309.1 | + | 4186 | 0.66 | 0.567563 |
Target: 5'- aCGAGGcAGCgcggaugucguccGCCagcUCGaACGCGCCgGUCGc -3' miRNA: 3'- -GCUCC-UUG-------------CGG---AGC-UGCGCGGgUAGC- -5' |
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12149 | 3' | -58.3 | NC_003309.1 | + | 3850 | 0.66 | 0.568616 |
Target: 5'- gGuuGAGCGCCU-GAUuCGCCCAUUGc -3' miRNA: 3'- gCucCUUGCGGAgCUGcGCGGGUAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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