Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12150 | 3' | -57 | NC_003309.1 | + | 43787 | 0.66 | 0.591049 |
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Target: 5'- ---aGCGCGCCCggcaaaCGucuCUCCGCGc -3' miRNA: 3'- uugaCGCGCGGGaaag--GCu--GAGGUGU- -5' |
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| 12150 | 3' | -57 | NC_003309.1 | + | 3928 | 0.66 | 0.569132 |
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Target: 5'- -uCUGCGCGCCgCUgucgaauucgCCGucCUCCAUg -3' miRNA: 3'- uuGACGCGCGG-GAaa--------GGCu-GAGGUGu -5' |
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| 12150 | 3' | -57 | NC_003309.1 | + | 26343 | 0.66 | 0.547422 |
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Target: 5'- cGAC-GCGCGCacgacgacggCCgcUCCGAUUCCACc -3' miRNA: 3'- -UUGaCGCGCG----------GGaaAGGCUGAGGUGu -5' |
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| 12150 | 3' | -57 | NC_003309.1 | + | 39351 | 0.66 | 0.547422 |
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Target: 5'- aAACUGCuuGgGCCgCUUUCCGAUUUCGg- -3' miRNA: 3'- -UUGACG--CgCGG-GAAAGGCUGAGGUgu -5' |
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| 12150 | 3' | -57 | NC_003309.1 | + | 19022 | 0.68 | 0.443923 |
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Target: 5'- -uCUGCGCGCCgUcgUCGAUcgCCGCAu -3' miRNA: 3'- uuGACGCGCGGgAaaGGCUGa-GGUGU- -5' |
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| 12150 | 3' | -57 | NC_003309.1 | + | 24039 | 0.68 | 0.42454 |
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Target: 5'- -cUUGCGUGCUCgacaCGGCUCCACc -3' miRNA: 3'- uuGACGCGCGGGaaagGCUGAGGUGu -5' |
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| 12150 | 3' | -57 | NC_003309.1 | + | 48685 | 0.69 | 0.396437 |
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Target: 5'- cGCUGCGCuGCgCUc-CCGAUUCCGCu -3' miRNA: 3'- uUGACGCG-CGgGAaaGGCUGAGGUGu -5' |
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| 12150 | 3' | -57 | NC_003309.1 | + | 26468 | 0.69 | 0.378379 |
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Target: 5'- cGGCUGCGcCGCCUUc-CCGuuACUCCAUAg -3' miRNA: 3'- -UUGACGC-GCGGGAaaGGC--UGAGGUGU- -5' |
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| 12150 | 3' | -57 | NC_003309.1 | + | 9462 | 0.71 | 0.296665 |
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Target: 5'- cGCUGUGCGCCCg--CCGucGCgcaCCGCAu -3' miRNA: 3'- uUGACGCGCGGGaaaGGC--UGa--GGUGU- -5' |
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| 12150 | 3' | -57 | NC_003309.1 | + | 32456 | 0.94 | 0.007025 |
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Target: 5'- gAACUGCGCGCCCUUcUCGACUCCACAc -3' miRNA: 3'- -UUGACGCGCGGGAAaGGCUGAGGUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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