Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12151 | 3' | -56.2 | NC_003309.1 | + | 10374 | 0.66 | 0.602597 |
Target: 5'- cGAGCGCC-GC-CUGCGCCGccuCGUg-- -3' miRNA: 3'- -UUCGUGGaCGaGAUGCGGCu--GCGuuu -5' |
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12151 | 3' | -56.2 | NC_003309.1 | + | 22368 | 0.66 | 0.584628 |
Target: 5'- -cGCgGCCUGCUCgucggcaagccgaugUGCGCCGAucaggugcucgaCGCAAGc -3' miRNA: 3'- uuCG-UGGACGAG---------------AUGCGGCU------------GCGUUU- -5' |
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12151 | 3' | -56.2 | NC_003309.1 | + | 20167 | 0.67 | 0.514167 |
Target: 5'- cGAGCACCUGCgauuCGaCGACGCc-- -3' miRNA: 3'- -UUCGUGGACGagauGCgGCUGCGuuu -5' |
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12151 | 3' | -56.2 | NC_003309.1 | + | 20277 | 0.67 | 0.482314 |
Target: 5'- -cGCACCga-UCaGCGCCuGACGCAAGa -3' miRNA: 3'- uuCGUGGacgAGaUGCGG-CUGCGUUU- -5' |
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12151 | 3' | -56.2 | NC_003309.1 | + | 18798 | 0.68 | 0.471909 |
Target: 5'- -cGCGCCUGCgcgaUGC-CCGACgGCAAGa -3' miRNA: 3'- uuCGUGGACGag--AUGcGGCUG-CGUUU- -5' |
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12151 | 3' | -56.2 | NC_003309.1 | + | 10946 | 0.69 | 0.421692 |
Target: 5'- cGAGCGCCg--UCUGCGCCGuCGCc-- -3' miRNA: 3'- -UUCGUGGacgAGAUGCGGCuGCGuuu -5' |
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12151 | 3' | -56.2 | NC_003309.1 | + | 45811 | 0.69 | 0.402524 |
Target: 5'- aGAGCACCgGCUCaagcACGCCcugcGCGCGAu -3' miRNA: 3'- -UUCGUGGaCGAGa---UGCGGc---UGCGUUu -5' |
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12151 | 3' | -56.2 | NC_003309.1 | + | 629 | 0.7 | 0.3571 |
Target: 5'- uGAGCcCCUGCUCUACGC--GCGgGAAa -3' miRNA: 3'- -UUCGuGGACGAGAUGCGgcUGCgUUU- -5' |
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12151 | 3' | -56.2 | NC_003309.1 | + | 31532 | 0.7 | 0.339965 |
Target: 5'- cAAGCGCCaGCUCgaacUACGCCGGC-CAGc -3' miRNA: 3'- -UUCGUGGaCGAG----AUGCGGCUGcGUUu -5' |
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12151 | 3' | -56.2 | NC_003309.1 | + | 35040 | 0.75 | 0.170597 |
Target: 5'- cGAGCGCCUGUgcgACGCCGACGaCGu- -3' miRNA: 3'- -UUCGUGGACGagaUGCGGCUGC-GUuu -5' |
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12151 | 3' | -56.2 | NC_003309.1 | + | 32571 | 1.04 | 0.001256 |
Target: 5'- cAAGCACCUGCUCUACGCCGACGCAAAg -3' miRNA: 3'- -UUCGUGGACGAGAUGCGGCUGCGUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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