miRNA display CGI


Results 1 - 3 of 3 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12154 5' -61.6 NC_003309.1 + 33649 1.08 0.00025
Target:  5'- gGCUGGCUGAGGCCGGCAUCGAGCCCUc -3'
miRNA:   3'- -CGACCGACUCCGGCCGUAGCUCGGGA- -5'
12154 5' -61.6 NC_003309.1 + 26862 0.69 0.23912
Target:  5'- cGCgGGCUGAagucgauccggggucGCCGaCAUCGAGCCCc -3'
miRNA:   3'- -CGaCCGACUc--------------CGGCcGUAGCUCGGGa -5'
12154 5' -61.6 NC_003309.1 + 519 0.66 0.385471
Target:  5'- uGCcGGCUc--GCCGG-GUCGAGCCCc -3'
miRNA:   3'- -CGaCCGAcucCGGCCgUAGCUCGGGa -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.