Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12156 | 5' | -56.2 | NC_003309.1 | + | 20042 | 0.66 | 0.607241 |
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Target: 5'- -aGGuCGGCgUACGacUUCGCGACGGUu- -3' miRNA: 3'- aaCCuGCCG-AUGU--GAGCGUUGCCGua -5' |
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| 12156 | 5' | -56.2 | NC_003309.1 | + | 46111 | 0.66 | 0.573781 |
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Target: 5'- -cGGuCGGCaccucgACACcgaaCGCGACGGCGa -3' miRNA: 3'- aaCCuGCCGa-----UGUGa---GCGUUGCCGUa -5' |
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| 12156 | 5' | -56.2 | NC_003309.1 | + | 4854 | 0.66 | 0.562716 |
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Target: 5'- --uGACGGCUGCGCguUCGCGGCGa--- -3' miRNA: 3'- aacCUGCCGAUGUG--AGCGUUGCcgua -5' |
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| 12156 | 5' | -56.2 | NC_003309.1 | + | 32388 | 0.66 | 0.562716 |
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Target: 5'- -gGcGACGGCU-CGCU-GCGGCGGCc- -3' miRNA: 3'- aaC-CUGCCGAuGUGAgCGUUGCCGua -5' |
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| 12156 | 5' | -56.2 | NC_003309.1 | + | 32778 | 0.67 | 0.551708 |
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Target: 5'- gUUGGACaGGCUcggacaggacaGCGCU-GCAGCaGGCGUg -3' miRNA: 3'- -AACCUG-CCGA-----------UGUGAgCGUUG-CCGUA- -5' |
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| 12156 | 5' | -56.2 | NC_003309.1 | + | 32595 | 0.67 | 0.540767 |
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Target: 5'- -cGGACGGCagACGCUgGgCAAaGGCAa -3' miRNA: 3'- aaCCUGCCGa-UGUGAgC-GUUgCCGUa -5' |
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| 12156 | 5' | -56.2 | NC_003309.1 | + | 32926 | 0.68 | 0.446399 |
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Target: 5'- -cGGcaagcaacaACGGCUAUGCggccggacugCGCGACGGCAg -3' miRNA: 3'- aaCC---------UGCCGAUGUGa---------GCGUUGCCGUa -5' |
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| 12156 | 5' | -56.2 | NC_003309.1 | + | 28034 | 0.69 | 0.417009 |
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Target: 5'- gUGGACGcGCUGCACguaUGCAgacguccaACGGCu- -3' miRNA: 3'- aACCUGC-CGAUGUGa--GCGU--------UGCCGua -5' |
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| 12156 | 5' | -56.2 | NC_003309.1 | + | 34732 | 1.03 | 0.001636 |
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Target: 5'- gUUGGACGGCUACACUCGCAACGGCAUc -3' miRNA: 3'- -AACCUGCCGAUGUGAGCGUUGCCGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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