Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12158 | 3' | -51.3 | NC_003309.1 | + | 44326 | 0.66 | 0.902351 |
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Target: 5'- cUCGUgacaacgUCggUGACGGCUgccgagaacgauUCGUUUUGCu -3' miRNA: 3'- uAGCG-------AGaaGCUGCCGA------------AGCAAAGCG- -5' |
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| 12158 | 3' | -51.3 | NC_003309.1 | + | 9719 | 0.66 | 0.895938 |
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Target: 5'- cGUCGCg--UCGAgCGGUUgcCGUgugUCGCg -3' miRNA: 3'- -UAGCGagaAGCU-GCCGAa-GCAa--AGCG- -5' |
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| 12158 | 3' | -51.3 | NC_003309.1 | + | 45958 | 0.66 | 0.895938 |
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Target: 5'- uGUCGCgca-UGACGcGCUUCGggaUCGCc -3' miRNA: 3'- -UAGCGagaaGCUGC-CGAAGCaa-AGCG- -5' |
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| 12158 | 3' | -51.3 | NC_003309.1 | + | 53030 | 0.66 | 0.888544 |
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Target: 5'- cGUCGgaCUUCcacuuGACGGCcUCGUg-CGCa -3' miRNA: 3'- -UAGCgaGAAG-----CUGCCGaAGCAaaGCG- -5' |
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| 12158 | 3' | -51.3 | NC_003309.1 | + | 1838 | 0.66 | 0.888544 |
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Target: 5'- uAUCGCUCcgccauaCGACGGuUUUCGguauugCGCa -3' miRNA: 3'- -UAGCGAGaa-----GCUGCC-GAAGCaaa---GCG- -5' |
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| 12158 | 3' | -51.3 | NC_003309.1 | + | 11484 | 0.66 | 0.880873 |
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Target: 5'- gAUCGCgc--CGGCGGC-UCGaacaagUUCGCa -3' miRNA: 3'- -UAGCGagaaGCUGCCGaAGCa-----AAGCG- -5' |
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| 12158 | 3' | -51.3 | NC_003309.1 | + | 49846 | 0.67 | 0.847548 |
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Target: 5'- -aCGUUCUUCGcgcGCGGCU-CGc-UCGCc -3' miRNA: 3'- uaGCGAGAAGC---UGCCGAaGCaaAGCG- -5' |
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| 12158 | 3' | -51.3 | NC_003309.1 | + | 9567 | 0.69 | 0.759606 |
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Target: 5'- -aCGCcgCUgCGGCGGCUugugCGUaUUCGCg -3' miRNA: 3'- uaGCGa-GAaGCUGCCGAa---GCA-AAGCG- -5' |
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| 12158 | 3' | -51.3 | NC_003309.1 | + | 4862 | 0.73 | 0.504338 |
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Target: 5'- -cCGCUgaUUGACGGCUgcgCGU-UCGCg -3' miRNA: 3'- uaGCGAgaAGCUGCCGAa--GCAaAGCG- -5' |
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| 12158 | 3' | -51.3 | NC_003309.1 | + | 35717 | 1.1 | 0.002076 |
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Target: 5'- aAUCGCUCUUCGACGGCUUCGUUUCGCu -3' miRNA: 3'- -UAGCGAGAAGCUGCCGAAGCAAAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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