miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12159 3' -49.5 NC_003309.1 + 27522 0.69 0.864629
Target:  5'- uGGAGCU--AUUCGcGCUCGACCa-- -3'
miRNA:   3'- uCCUUGAagUGAGCaUGAGCUGGaag -5'
12159 3' -49.5 NC_003309.1 + 11705 0.69 0.83811
Target:  5'- ------cUCGcCUCGUAUUCGGCCUUCu -3'
miRNA:   3'- uccuugaAGU-GAGCAUGAGCUGGAAG- -5'
12159 3' -49.5 NC_003309.1 + 31611 0.71 0.736129
Target:  5'- uGGAGcCUUUGCUCGgGCcggUUGACCUUCg -3'
miRNA:   3'- uCCUU-GAAGUGAGCaUG---AGCUGGAAG- -5'
12159 3' -49.5 NC_003309.1 + 36486 1.12 0.002574
Target:  5'- gAGGAACUUCACUCGUACUCGACCUUCg -3'
miRNA:   3'- -UCCUUGAAGUGAGCAUGAGCUGGAAG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.