miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12159 5' -56.1 NC_003309.1 + 3305 0.66 0.653506
Target:  5'- gGAAAGgcgcAGCGCGUCGAuca-GCGCUGc -3'
miRNA:   3'- aCUUUC----UCGCGCAGUUccggCGCGAC- -5'
12159 5' -56.1 NC_003309.1 + 6042 0.66 0.653506
Target:  5'- cGAAuca--GCGUCu-GGUCGCGCUGg -3'
miRNA:   3'- aCUUucucgCGCAGuuCCGGCGCGAC- -5'
12159 5' -56.1 NC_003309.1 + 11231 0.68 0.511523
Target:  5'- cGGAcu-GCGCGUCGucuGCCGCGCg- -3'
miRNA:   3'- aCUUucuCGCGCAGUuc-CGGCGCGac -5'
12159 5' -56.1 NC_003309.1 + 34398 0.65 0.661245
Target:  5'- gUGAAcgcaucaaagccuuGGAGCGCGaggUCAAGGaaCUGCGCc- -3'
miRNA:   3'- -ACUU--------------UCUCGCGC---AGUUCC--GGCGCGac -5'
12159 5' -56.1 NC_003309.1 + 36450 1.08 0.000893
Target:  5'- uUGAAAGAGCGCGUCAAGGCCGCGCUGc -3'
miRNA:   3'- -ACUUUCUCGCGCAGUUCCGGCGCGAC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.