Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1216 | 5' | -47.4 | NC_001266.1 | + | 29984 | 0.66 | 0.99981 |
Target: 5'- ---gACUCUuucuauCUCGuGGAUGUGUCCGu -3' miRNA: 3'- cuuaUGAGG------GAGCuCUUACAUAGGUc -5' |
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1216 | 5' | -47.4 | NC_001266.1 | + | 1726 | 0.66 | 0.999689 |
Target: 5'- ---aACUCCUUCGuuuuGAucacuucguUGUGUCCAGc -3' miRNA: 3'- cuuaUGAGGGAGCu---CUu--------ACAUAGGUC- -5' |
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1216 | 5' | -47.4 | NC_001266.1 | + | 158132 | 0.66 | 0.999689 |
Target: 5'- ---aACUCCUUCGuuuuGAucacuucguUGUGUCCAGc -3' miRNA: 3'- cuuaUGAGGGAGCu---CUu--------ACAUAGGUC- -5' |
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1216 | 5' | -47.4 | NC_001266.1 | + | 139028 | 0.67 | 0.999058 |
Target: 5'- ---cGCUUCCUCGGGugucAUGUGUUCGu -3' miRNA: 3'- cuuaUGAGGGAGCUCu---UACAUAGGUc -5' |
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1216 | 5' | -47.4 | NC_001266.1 | + | 14705 | 0.68 | 0.998846 |
Target: 5'- aAAUACUCCCacgCGGGAggAUGU-UCCAc -3' miRNA: 3'- cUUAUGAGGGa--GCUCU--UACAuAGGUc -5' |
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1216 | 5' | -47.4 | NC_001266.1 | + | 1891 | 0.7 | 0.991311 |
Target: 5'- aGAGUAuCUCCCgcUCGc--GUGUGUCCAGa -3' miRNA: 3'- -CUUAU-GAGGG--AGCucuUACAUAGGUC- -5' |
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1216 | 5' | -47.4 | NC_001266.1 | + | 157967 | 0.7 | 0.991311 |
Target: 5'- aGAGUAuCUCCCgcUCGc--GUGUGUCCAGa -3' miRNA: 3'- -CUUAU-GAGGG--AGCucuUACAUAGGUC- -5' |
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1216 | 5' | -47.4 | NC_001266.1 | + | 52236 | 1.11 | 0.016209 |
Target: 5'- aGAAUACUCCCUCGAGAAUGUAUCCAGa -3' miRNA: 3'- -CUUAUGAGGGAGCUCUUACAUAGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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