Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12161 | 5' | -58.2 | NC_003309.1 | + | 37303 | 1.09 | 0.000523 |
Target: 5'- cGUGCGCGACGCCCUGAAUCUCGCCGGc -3' miRNA: 3'- -CACGCGCUGCGGGACUUAGAGCGGCC- -5' |
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12161 | 5' | -58.2 | NC_003309.1 | + | 18793 | 0.74 | 0.204095 |
Target: 5'- cUGCGCGAUGCCCgacgGcaaGAUCgccgugaaacgaUCGCCGGc -3' miRNA: 3'- cACGCGCUGCGGGa---C---UUAG------------AGCGGCC- -5' |
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12161 | 5' | -58.2 | NC_003309.1 | + | 10684 | 0.72 | 0.270085 |
Target: 5'- cUGCGCGGCGgCC---GUCUCGCgGGc -3' miRNA: 3'- cACGCGCUGCgGGacuUAGAGCGgCC- -5' |
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12161 | 5' | -58.2 | NC_003309.1 | + | 32950 | 0.71 | 0.298032 |
Target: 5'- -aGCGCGACGaCCCUGAGcugaUCGCgGc -3' miRNA: 3'- caCGCGCUGC-GGGACUUag--AGCGgCc -5' |
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12161 | 5' | -58.2 | NC_003309.1 | + | 31696 | 0.71 | 0.298032 |
Target: 5'- -gGCGCGACGaUCgUGAAUCagGCCGa -3' miRNA: 3'- caCGCGCUGC-GGgACUUAGagCGGCc -5' |
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12161 | 5' | -58.2 | NC_003309.1 | + | 10824 | 0.71 | 0.298032 |
Target: 5'- -cGCGCGAgGCCgUGAuccGUCUCgGCCa- -3' miRNA: 3'- caCGCGCUgCGGgACU---UAGAG-CGGcc -5' |
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12161 | 5' | -58.2 | NC_003309.1 | + | 18748 | 0.71 | 0.303883 |
Target: 5'- -gGCGauaguCGGCGCCUUGucgagcgugaucGUCUCGCCGGc -3' miRNA: 3'- caCGC-----GCUGCGGGACu-----------UAGAGCGGCC- -5' |
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12161 | 5' | -58.2 | NC_003309.1 | + | 5764 | 0.7 | 0.352214 |
Target: 5'- cGUGCGCGGgGCCaUGAucca-GCCGGg -3' miRNA: 3'- -CACGCGCUgCGGgACUuagagCGGCC- -5' |
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12161 | 5' | -58.2 | NC_003309.1 | + | 536 | 0.69 | 0.413015 |
Target: 5'- uUGCGcCGACcagGCgCUGccggCUCGCCGGg -3' miRNA: 3'- cACGC-GCUG---CGgGACuua-GAGCGGCC- -5' |
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12161 | 5' | -58.2 | NC_003309.1 | + | 16905 | 0.69 | 0.374042 |
Target: 5'- -aGUGCGGCGCCgaGAAUCguguugaagagccCGCCGc -3' miRNA: 3'- caCGCGCUGCGGgaCUUAGa------------GCGGCc -5' |
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12161 | 5' | -58.2 | NC_003309.1 | + | 22427 | 0.68 | 0.422217 |
Target: 5'- -aGCGCGGCGCgCgaGAUCUUGCCc- -3' miRNA: 3'- caCGCGCUGCGgGacUUAGAGCGGcc -5' |
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12161 | 5' | -58.2 | NC_003309.1 | + | 22386 | 0.68 | 0.425931 |
Target: 5'- cUGCGCGucgaACGCCCgcgcggccugCUCGUCGGc -3' miRNA: 3'- cACGCGC----UGCGGGacuua-----GAGCGGCC- -5' |
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12161 | 5' | -58.2 | NC_003309.1 | + | 11525 | 0.67 | 0.479878 |
Target: 5'- aGUGCuuGCG-CGCCCUucugcgacgcGAggCUCGCCGu -3' miRNA: 3'- -CACG--CGCuGCGGGA----------CUuaGAGCGGCc -5' |
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12161 | 5' | -58.2 | NC_003309.1 | + | 34377 | 0.67 | 0.479878 |
Target: 5'- -aGCGCGAgGUCaaGGAaCUgCGCCGGa -3' miRNA: 3'- caCGCGCUgCGGgaCUUaGA-GCGGCC- -5' |
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12161 | 5' | -58.2 | NC_003309.1 | + | 13809 | 0.67 | 0.527604 |
Target: 5'- uGUGUGCGACGCgguuguugucgccgCCgcu-UC-CGCCGGg -3' miRNA: 3'- -CACGCGCUGCG--------------GGacuuAGaGCGGCC- -5' |
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12161 | 5' | -58.2 | NC_003309.1 | + | 16079 | 0.67 | 0.530714 |
Target: 5'- uGUGC-CGACGCCCccggaugcgaaUGcacgagagcgacGAUCUCGCCa- -3' miRNA: 3'- -CACGcGCUGCGGG-----------AC------------UUAGAGCGGcc -5' |
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12161 | 5' | -58.2 | NC_003309.1 | + | 27385 | 0.66 | 0.541126 |
Target: 5'- uGUG-GCGaACGCCac---UCUCGCCGGc -3' miRNA: 3'- -CACgCGC-UGCGGgacuuAGAGCGGCC- -5' |
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12161 | 5' | -58.2 | NC_003309.1 | + | 21895 | 0.66 | 0.541126 |
Target: 5'- -cGUGaCGAUGCCgaUUGGAUCUgGCCGc -3' miRNA: 3'- caCGC-GCUGCGG--GACUUAGAgCGGCc -5' |
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12161 | 5' | -58.2 | NC_003309.1 | + | 23972 | 0.65 | 0.591918 |
Target: 5'- -cGcCGCGACGaucggugcCCCUGAAUacgucaacuugaUCGCUGGa -3' miRNA: 3'- caC-GCGCUGC--------GGGACUUAg-----------AGCGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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