Results 1 - 20 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12162 | 5' | -54.7 | NC_003309.1 | + | 38116 | 0.66 | 0.73857 |
Target: 5'- gCGGCAauUGCUcCGCG-CGCAGUUCUUu -3' miRNA: 3'- aGUCGU--ACGGuGUGUgGCGUCAAGGG- -5' |
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12162 | 5' | -54.7 | NC_003309.1 | + | 44573 | 0.66 | 0.71713 |
Target: 5'- cCGGC-UGCCGCagacgaucGCGCUGCAGcggCUCa -3' miRNA: 3'- aGUCGuACGGUG--------UGUGGCGUCaa-GGG- -5' |
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12162 | 5' | -54.7 | NC_003309.1 | + | 10480 | 0.66 | 0.71713 |
Target: 5'- uUCGcGCAUcGCgCGCAUGCCGCgAGUUCggCCu -3' miRNA: 3'- -AGU-CGUA-CG-GUGUGUGGCG-UCAAG--GG- -5' |
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12162 | 5' | -54.7 | NC_003309.1 | + | 49097 | 0.66 | 0.706275 |
Target: 5'- uUCGGCGUGCCG---ACC-CGGUUCUg -3' miRNA: 3'- -AGUCGUACGGUgugUGGcGUCAAGGg -5' |
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12162 | 5' | -54.7 | NC_003309.1 | + | 3938 | 0.66 | 0.706275 |
Target: 5'- gCGGCA-GCCaucuGCGCGCCGCuGUcgaauUCgCCg -3' miRNA: 3'- aGUCGUaCGG----UGUGUGGCGuCA-----AG-GG- -5' |
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12162 | 5' | -54.7 | NC_003309.1 | + | 27126 | 0.66 | 0.706275 |
Target: 5'- ---cCAUGCuuuccgcucgCACGCGCCGCAGUgCCUu -3' miRNA: 3'- agucGUACG----------GUGUGUGGCGUCAaGGG- -5' |
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12162 | 5' | -54.7 | NC_003309.1 | + | 47380 | 0.66 | 0.706275 |
Target: 5'- -gGGCA-GCUcgAUugAUCGCAGUUCgCCa -3' miRNA: 3'- agUCGUaCGG--UGugUGGCGUCAAG-GG- -5' |
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12162 | 5' | -54.7 | NC_003309.1 | + | 6515 | 0.66 | 0.695346 |
Target: 5'- cUCGGCcgcuUGCCGCGCGCC-CGGgaUCa -3' miRNA: 3'- -AGUCGu---ACGGUGUGUGGcGUCaaGGg -5' |
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12162 | 5' | -54.7 | NC_003309.1 | + | 43235 | 0.66 | 0.695346 |
Target: 5'- --cGCGUaaCCACGCGCCGCug--CCCa -3' miRNA: 3'- aguCGUAc-GGUGUGUGGCGucaaGGG- -5' |
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12162 | 5' | -54.7 | NC_003309.1 | + | 497 | 0.67 | 0.684354 |
Target: 5'- cCAGCAUugcgccgaagccGCCAUuuGCGCCGCcGcUUCCUu -3' miRNA: 3'- aGUCGUA------------CGGUG--UGUGGCGuC-AAGGG- -5' |
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12162 | 5' | -54.7 | NC_003309.1 | + | 13462 | 0.67 | 0.672204 |
Target: 5'- -gGGCuuUGCCACACgcagcgucguaucGCCGCcGUgCCCu -3' miRNA: 3'- agUCGu-ACGGUGUG-------------UGGCGuCAaGGG- -5' |
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12162 | 5' | -54.7 | NC_003309.1 | + | 44717 | 0.67 | 0.651117 |
Target: 5'- cCGGUAUccGCCAuCACAgcCCGCAcGUUCuCCg -3' miRNA: 3'- aGUCGUA--CGGU-GUGU--GGCGU-CAAG-GG- -5' |
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12162 | 5' | -54.7 | NC_003309.1 | + | 5838 | 0.67 | 0.639987 |
Target: 5'- uUCAGCAUGCCGUugG-CG-AGUUCCg -3' miRNA: 3'- -AGUCGUACGGUGugUgGCgUCAAGGg -5' |
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12162 | 5' | -54.7 | NC_003309.1 | + | 12554 | 0.68 | 0.628851 |
Target: 5'- -aAGCGUGCCguccuuucGCGCGCCcuucguCAGUucgUCCCg -3' miRNA: 3'- agUCGUACGG--------UGUGUGGc-----GUCA---AGGG- -5' |
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12162 | 5' | -54.7 | NC_003309.1 | + | 17577 | 0.68 | 0.628851 |
Target: 5'- cCGGCAcGCCgaagcgcacACGCACCGCGGUcagUUgCg -3' miRNA: 3'- aGUCGUaCGG---------UGUGUGGCGUCA---AGgG- -5' |
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12162 | 5' | -54.7 | NC_003309.1 | + | 32510 | 0.68 | 0.606596 |
Target: 5'- aUCAGCAgagcUGCCgACACGCUGgAaUUCCa -3' miRNA: 3'- -AGUCGU----ACGG-UGUGUGGCgUcAAGGg -5' |
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12162 | 5' | -54.7 | NC_003309.1 | + | 23356 | 0.68 | 0.595498 |
Target: 5'- gCAGCAgguucagGCCcgGCACCGUAGUcgUCgCCg -3' miRNA: 3'- aGUCGUa------CGGugUGUGGCGUCA--AG-GG- -5' |
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12162 | 5' | -54.7 | NC_003309.1 | + | 6383 | 0.68 | 0.592174 |
Target: 5'- aCAGCA-GCCAUACcgcguuaccccuucGCCGCGGUgggCUUg -3' miRNA: 3'- aGUCGUaCGGUGUG--------------UGGCGUCAa--GGG- -5' |
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12162 | 5' | -54.7 | NC_003309.1 | + | 53057 | 0.68 | 0.572306 |
Target: 5'- cUCGcGCAugacgauUGCCGCGCACCGC-GUcggacUUCCa -3' miRNA: 3'- -AGU-CGU-------ACGGUGUGUGGCGuCA-----AGGG- -5' |
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12162 | 5' | -54.7 | NC_003309.1 | + | 22398 | 0.69 | 0.562429 |
Target: 5'- uUCGGCAUGCCGCugcgcgucgaACGcCCGCGcggCCUg -3' miRNA: 3'- -AGUCGUACGGUG----------UGU-GGCGUcaaGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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