Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12167 | 5' | -59.4 | NC_003309.1 | + | 27367 | 0.66 | 0.462896 |
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Target: 5'- cGCCGGCCaccuaCUGCCCGCccaaaACGGGAGc- -3' miRNA: 3'- cUGGUUGG-----GGCGGGCGa----UGUCCUCuu -5' |
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| 12167 | 5' | -59.4 | NC_003309.1 | + | 33450 | 0.66 | 0.433732 |
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Target: 5'- cGGCUAuAUCCCGCCCGCUGaAGcuGAGGc -3' miRNA: 3'- -CUGGU-UGGGGCGGGCGAUgUC--CUCUu -5' |
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| 12167 | 5' | -59.4 | NC_003309.1 | + | 32185 | 0.68 | 0.320816 |
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Target: 5'- cGCCGACgCCGCCCGCaccCAaGAGAGc -3' miRNA: 3'- cUGGUUGgGGCGGGCGau-GUcCUCUU- -5' |
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| 12167 | 5' | -59.4 | NC_003309.1 | + | 24283 | 0.69 | 0.3131 |
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Target: 5'- uGAUCGACCgCCGCCUGCUucuCGGGuGu- -3' miRNA: 3'- -CUGGUUGG-GGCGGGCGAu--GUCCuCuu -5' |
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| 12167 | 5' | -59.4 | NC_003309.1 | + | 39185 | 0.7 | 0.243643 |
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Target: 5'- aACCAACCgCGCCCGCccUGCGGGc--- -3' miRNA: 3'- cUGGUUGGgGCGGGCG--AUGUCCucuu -5' |
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| 12167 | 5' | -59.4 | NC_003309.1 | + | 39768 | 0.97 | 0.002659 |
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Target: 5'- uGACCAACCgCGCCCGCUACAGGAGAAa -3' miRNA: 3'- -CUGGUUGGgGCGGGCGAUGUCCUCUU- -5' |
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| 12167 | 5' | -59.4 | NC_003309.1 | + | 39561 | 1.05 | 0.000623 |
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Target: 5'- uGACCAACCCCGCCCGCUACAGGAGAAc -3' miRNA: 3'- -CUGGUUGGGGCGGGCGAUGUCCUCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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