Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12168 | 3' | -63.2 | NC_003309.1 | + | 668 | 0.66 | 0.321102 |
Target: 5'- -aCGCAGGGcgcuugccacgaacGACGUCUCGCGCgaACUUg -3' miRNA: 3'- ggGCGUCCCa-------------CUGCGGGGCGCG--UGAG- -5' |
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12168 | 3' | -63.2 | NC_003309.1 | + | 38120 | 0.67 | 0.30358 |
Target: 5'- uCCCGCGGcaau-UGCUCCGCGCGCa- -3' miRNA: 3'- -GGGCGUCccacuGCGGGGCGCGUGag -5' |
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12168 | 3' | -63.2 | NC_003309.1 | + | 13274 | 0.69 | 0.21057 |
Target: 5'- gCUCGCAGGGUucGAcgaucCGCCCUcgaucgaaacugGCGCGCUg -3' miRNA: 3'- -GGGCGUCCCA--CU-----GCGGGG------------CGCGUGAg -5' |
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12168 | 3' | -63.2 | NC_003309.1 | + | 47658 | 0.74 | 0.098672 |
Target: 5'- aCCGCGGucaGUGACaCCuuGCGCGCUCu -3' miRNA: 3'- gGGCGUCc--CACUGcGGggCGCGUGAG- -5' |
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12168 | 3' | -63.2 | NC_003309.1 | + | 41085 | 1.09 | 0.000176 |
Target: 5'- cCCCGCAGGGUGACGCCCCGCGCACUCc -3' miRNA: 3'- -GGGCGUCCCACUGCGGGGCGCGUGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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