Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12169 | 5' | -51.3 | NC_003309.1 | + | 5066 | 0.66 | 0.880214 |
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Target: 5'- -cACGGgCGCGGCGGccGGUGCcgCGAc -3' miRNA: 3'- gaUGCCgGCGUCGUUu-UUACGa-GCUa -5' |
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| 12169 | 5' | -51.3 | NC_003309.1 | + | 29812 | 0.66 | 0.872092 |
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Target: 5'- -cGCGauCCGCAGCGc-GAUGCUUGAg -3' miRNA: 3'- gaUGCc-GGCGUCGUuuUUACGAGCUa -5' |
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| 12169 | 5' | -51.3 | NC_003309.1 | + | 31202 | 0.67 | 0.79701 |
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Target: 5'- -gACGGCCGCAGgCAcgacgaauuccccGAGGUGacgCGAUg -3' miRNA: 3'- gaUGCCGGCGUC-GU-------------UUUUACga-GCUA- -5' |
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| 12169 | 5' | -51.3 | NC_003309.1 | + | 44268 | 0.68 | 0.75605 |
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Target: 5'- -cACGagcGCCGCGGUu---GUGCUCGAc -3' miRNA: 3'- gaUGC---CGGCGUCGuuuuUACGAGCUa -5' |
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| 12169 | 5' | -51.3 | NC_003309.1 | + | 46497 | 0.69 | 0.67774 |
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Target: 5'- --uUGGCCGuCGGCGGGAAcUGCUCGc- -3' miRNA: 3'- gauGCCGGC-GUCGUUUUU-ACGAGCua -5' |
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| 12169 | 5' | -51.3 | NC_003309.1 | + | 10428 | 0.7 | 0.616639 |
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Target: 5'- cCUGCGcguuuugcagcuucGCCGCAGCAGca--GCUCGAUc -3' miRNA: 3'- -GAUGC--------------CGGCGUCGUUuuuaCGAGCUA- -5' |
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| 12169 | 5' | -51.3 | NC_003309.1 | + | 40090 | 0.71 | 0.585564 |
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Target: 5'- aUGCGGCaCGCGGCccgguuGAA-GCUCGAa -3' miRNA: 3'- gAUGCCG-GCGUCGuu----UUUaCGAGCUa -5' |
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| 12169 | 5' | -51.3 | NC_003309.1 | + | 39371 | 0.72 | 0.55141 |
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Target: 5'- cCUuCGGCCGCAuucccGCAAAAcUGCUUGGg -3' miRNA: 3'- -GAuGCCGGCGU-----CGUUUUuACGAGCUa -5' |
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| 12169 | 5' | -51.3 | NC_003309.1 | + | 52465 | 0.76 | 0.327484 |
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Target: 5'- -gACGGCCGCcGCAucccagccgcuGUGCUCGAUg -3' miRNA: 3'- gaUGCCGGCGuCGUuuu--------UACGAGCUA- -5' |
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| 12169 | 5' | -51.3 | NC_003309.1 | + | 41503 | 1.07 | 0.002722 |
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Target: 5'- cCUACGGCCGCAGCAAAAAUGCUCGAUa -3' miRNA: 3'- -GAUGCCGGCGUCGUUUUUACGAGCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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