Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1217 | 5' | -43.4 | NC_001266.1 | + | 83836 | 0.66 | 1 |
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Target: 5'- uUGAACUCgGGuguUCuUUGUUCUAGa -3' miRNA: 3'- cAUUUGAGgCCuuuAGcAACAAGAUU- -5' |
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| 1217 | 5' | -43.4 | NC_001266.1 | + | 52496 | 0.68 | 0.999995 |
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Target: 5'- cGUGGAgUCgGGAccugGAUCGUUGUUUa-- -3' miRNA: 3'- -CAUUUgAGgCCU----UUAGCAACAAGauu -5' |
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| 1217 | 5' | -43.4 | NC_001266.1 | + | 119139 | 0.7 | 0.999944 |
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Target: 5'- -aAGACUCCGGAAAgugCGUUaagcgUCUGu -3' miRNA: 3'- caUUUGAGGCCUUUa--GCAAca---AGAUu -5' |
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| 1217 | 5' | -43.4 | NC_001266.1 | + | 132744 | 0.7 | 0.999859 |
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Target: 5'- -cAGACUCCGGGuaaaacauacguAUCGUUGUUUUu- -3' miRNA: 3'- caUUUGAGGCCUu-----------UAGCAACAAGAuu -5' |
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| 1217 | 5' | -43.4 | NC_001266.1 | + | 92412 | 0.71 | 0.999773 |
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Target: 5'- aUAAACUUuugCGGAuucaAGUCGUUGUUUUGAa -3' miRNA: 3'- cAUUUGAG---GCCU----UUAGCAACAAGAUU- -5' |
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| 1217 | 5' | -43.4 | NC_001266.1 | + | 52652 | 1.07 | 0.063566 |
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Target: 5'- uGUAAACUCCGGAAAUCGUUGUUCUAAu -3' miRNA: 3'- -CAUUUGAGGCCUUUAGCAACAAGAUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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