Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12173 | 3' | -60.9 | NC_003309.1 | + | 51984 | 0.68 | 0.326166 |
Target: 5'- gACCUCGGAGU--UCGACGuCGCUgG-Ca -3' miRNA: 3'- -UGGAGCCUCAcgAGCUGC-GCGGgCaG- -5' |
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12173 | 3' | -60.9 | NC_003309.1 | + | 45407 | 0.66 | 0.431368 |
Target: 5'- gACUUCGGAcacgagacGUcccauuccgcaaugcGCUCGACGUGCUCGg- -3' miRNA: 3'- -UGGAGCCU--------CA---------------CGAGCUGCGCGGGCag -5' |
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12173 | 3' | -60.9 | NC_003309.1 | + | 44262 | 0.73 | 0.157201 |
Target: 5'- cGCCgCGGuuGUGCUCGACagucgaauguGCGCCCGg- -3' miRNA: 3'- -UGGaGCCu-CACGAGCUG----------CGCGGGCag -5' |
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12173 | 3' | -60.9 | NC_003309.1 | + | 43619 | 0.72 | 0.174646 |
Target: 5'- gAUCUCGcGGUGCUUGuccGgGUGCCCGUCg -3' miRNA: 3'- -UGGAGCcUCACGAGC---UgCGCGGGCAG- -5' |
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12173 | 3' | -60.9 | NC_003309.1 | + | 43376 | 1.08 | 0.000327 |
Target: 5'- gACCUCGGAGUGCUCGACGCGCCCGUCu -3' miRNA: 3'- -UGGAGCCUCACGAGCUGCGCGGGCAG- -5' |
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12173 | 3' | -60.9 | NC_003309.1 | + | 28339 | 0.69 | 0.262516 |
Target: 5'- aACCcCGcGAGgaacGcCUCGACGCGCCCaUCg -3' miRNA: 3'- -UGGaGC-CUCa---C-GAGCUGCGCGGGcAG- -5' |
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12173 | 3' | -60.9 | NC_003309.1 | + | 24265 | 0.72 | 0.161408 |
Target: 5'- uUCUCGG-GUGUgUCGAUGUaGCCCGUCa -3' miRNA: 3'- uGGAGCCuCACG-AGCUGCG-CGGGCAG- -5' |
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12173 | 3' | -60.9 | NC_003309.1 | + | 16086 | 0.66 | 0.427673 |
Target: 5'- -gCUCGGcuGUGC-CGACGCcCCCGg- -3' miRNA: 3'- ugGAGCCu-CACGaGCUGCGcGGGCag -5' |
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12173 | 3' | -60.9 | NC_003309.1 | + | 12605 | 0.69 | 0.282538 |
Target: 5'- cGCCUCGGccGUGUU-GAUGCGCUCGaUCu -3' miRNA: 3'- -UGGAGCCu-CACGAgCUGCGCGGGC-AG- -5' |
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12173 | 3' | -60.9 | NC_003309.1 | + | 11532 | 0.69 | 0.269059 |
Target: 5'- cGCCgu-GAGUGCUUG-CGCGCCCuUCu -3' miRNA: 3'- -UGGagcCUCACGAGCuGCGCGGGcAG- -5' |
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12173 | 3' | -60.9 | NC_003309.1 | + | 10708 | 0.68 | 0.326166 |
Target: 5'- aGCCUCGGcGcGCUCGAgcuccugcUGCGCggcggCCGUCu -3' miRNA: 3'- -UGGAGCCuCaCGAGCU--------GCGCG-----GGCAG- -5' |
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12173 | 3' | -60.9 | NC_003309.1 | + | 6519 | 0.66 | 0.409487 |
Target: 5'- uGCuCUCGGcc-GCUUGccGCGCGCCCGg- -3' miRNA: 3'- -UG-GAGCCucaCGAGC--UGCGCGGGCag -5' |
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12173 | 3' | -60.9 | NC_003309.1 | + | 5609 | 0.76 | 0.094137 |
Target: 5'- cCUUCGGGGUGUUCGcCGUGCCguCGUCg -3' miRNA: 3'- uGGAGCCUCACGAGCuGCGCGG--GCAG- -5' |
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12173 | 3' | -60.9 | NC_003309.1 | + | 4182 | 0.66 | 0.436944 |
Target: 5'- gGCagcgCGGAuGUcguccgccaGCUCGaACGCGCCgGUCg -3' miRNA: 3'- -UGga--GCCU-CA---------CGAGC-UGCGCGGgCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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