Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12174 | 5' | -59 | NC_003309.1 | + | 35648 | 0.66 | 0.50999 |
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Target: 5'- aGCGcGACGaCCAaGGCUGGCUgacgCAUCc -3' miRNA: 3'- gCGUuCUGC-GGUgCCGACCGG----GUAGu -5' |
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| 12174 | 5' | -59 | NC_003309.1 | + | 40157 | 0.66 | 0.459717 |
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Target: 5'- cCGUGAGACGUgGgCGGUacucGGCCUAUCu -3' miRNA: 3'- -GCGUUCUGCGgU-GCCGa---CCGGGUAGu -5' |
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| 12174 | 5' | -59 | NC_003309.1 | + | 41034 | 0.67 | 0.45386 |
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Target: 5'- cCGuCGGGACGCCGCcGCUGcacaagcgcgacagcGCCCAaCAg -3' miRNA: 3'- -GC-GUUCUGCGGUGcCGAC---------------CGGGUaGU- -5' |
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| 12174 | 5' | -59 | NC_003309.1 | + | 40394 | 0.67 | 0.430845 |
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Target: 5'- aGCcGGAaGUCGCGGCagUGGCcCCAUCc -3' miRNA: 3'- gCGuUCUgCGGUGCCG--ACCG-GGUAGu -5' |
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| 12174 | 5' | -59 | NC_003309.1 | + | 44270 | 0.68 | 0.36784 |
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Target: 5'- uGCAcGAgCGCCGCGGUUGuGCUCGaCAg -3' miRNA: 3'- gCGUuCU-GCGGUGCCGAC-CGGGUaGU- -5' |
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| 12174 | 5' | -59 | NC_003309.1 | + | 52581 | 0.68 | 0.36784 |
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Target: 5'- aGCAGGACgGCCACcgGGCcuuugccgUGGucCCCAUCGa -3' miRNA: 3'- gCGUUCUG-CGGUG--CCG--------ACC--GGGUAGU- -5' |
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| 12174 | 5' | -59 | NC_003309.1 | + | 11985 | 0.68 | 0.351038 |
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Target: 5'- gCGCGcGGCaGCCGCGGCgcgcGCCgCGUCGg -3' miRNA: 3'- -GCGUuCUG-CGGUGCCGac--CGG-GUAGU- -5' |
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| 12174 | 5' | -59 | NC_003309.1 | + | 43881 | 1.09 | 0.000391 |
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Target: 5'- aCGCAAGACGCCACGGCUGGCCCAUCAg -3' miRNA: 3'- -GCGUUCUGCGGUGCCGACCGGGUAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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