Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12178 | 3' | -57.2 | NC_003309.1 | + | 29950 | 0.66 | 0.593711 |
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Target: 5'- aCGCGUCaUGCGCGCGuuccugUCCGGu -3' miRNA: 3'- -GUGCGGgACGCGCGCuauca-AGGCUu -5' |
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| 12178 | 3' | -57.2 | NC_003309.1 | + | 19964 | 0.66 | 0.55025 |
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Target: 5'- uGCG-CCUGCGCGCGAUccgcUUCGAc -3' miRNA: 3'- gUGCgGGACGCGCGCUAuca-AGGCUu -5' |
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| 12178 | 3' | -57.2 | NC_003309.1 | + | 9963 | 0.68 | 0.466831 |
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Target: 5'- cCAUGCC--GCGCGCGAUAGcaUCCGc- -3' miRNA: 3'- -GUGCGGgaCGCGCGCUAUCa-AGGCuu -5' |
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| 12178 | 3' | -57.2 | NC_003309.1 | + | 37296 | 0.68 | 0.466831 |
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Target: 5'- gACGCCCUgaaucucgccgGCGCGuCGAUGGcggUCGAGg -3' miRNA: 3'- gUGCGGGA-----------CGCGC-GCUAUCaa-GGCUU- -5' |
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| 12178 | 3' | -57.2 | NC_003309.1 | + | 33032 | 0.69 | 0.408761 |
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Target: 5'- gACGaCCUGaucaacCGCGCGAUcGUUCCGAc -3' miRNA: 3'- gUGCgGGAC------GCGCGCUAuCAAGGCUu -5' |
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| 12178 | 3' | -57.2 | NC_003309.1 | + | 45796 | 1.05 | 0.001039 |
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Target: 5'- gCACGCCCUGCGCGCGAUAGUUCCGAAc -3' miRNA: 3'- -GUGCGGGACGCGCGCUAUCAAGGCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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