Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12178 | 5' | -56.8 | NC_003309.1 | + | 45761 | 1.12 | 0.000549 |
Target: 5'- gGGUUCGGCGCCUGAUGUACGCGCCGGu -3' miRNA: 3'- -CCAAGCCGCGGACUACAUGCGCGGCC- -5' |
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12178 | 5' | -56.8 | NC_003309.1 | + | 3958 | 0.7 | 0.411281 |
Target: 5'- aGGcacgUCGGCGCUccGAUGcggcagccaucUGCGCGCCGc -3' miRNA: 3'- -CCa---AGCCGCGGa-CUAC-----------AUGCGCGGCc -5' |
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12178 | 5' | -56.8 | NC_003309.1 | + | 7764 | 0.69 | 0.47804 |
Target: 5'- cGGcgcCGGCGCCUucgccgGUGCGaCGCCGa -3' miRNA: 3'- -CCaa-GCCGCGGAcua---CAUGC-GCGGCc -5' |
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12178 | 5' | -56.8 | NC_003309.1 | + | 11799 | 0.68 | 0.560247 |
Target: 5'- --gUCGGCgaucGCCUuuucgagcGAcuUGUACGCGUCGGc -3' miRNA: 3'- ccaAGCCG----CGGA--------CU--ACAUGCGCGGCC- -5' |
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12178 | 5' | -56.8 | NC_003309.1 | + | 10649 | 0.67 | 0.570839 |
Target: 5'- cGUaCGGCauGCCcgcGAUGcGCGCGCCGa -3' miRNA: 3'- cCAaGCCG--CGGa--CUACaUGCGCGGCc -5' |
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12178 | 5' | -56.8 | NC_003309.1 | + | 4204 | 0.66 | 0.632964 |
Target: 5'- cGGUgaUCGGCuucaccaacgaggcaGCgCgGAUGUcguccgccagcucgaACGCGCCGGu -3' miRNA: 3'- -CCA--AGCCG---------------CG-GaCUACA---------------UGCGCGGCC- -5' |
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12178 | 5' | -56.8 | NC_003309.1 | + | 13239 | 0.66 | 0.642645 |
Target: 5'- ---cUGGCGCgCUGAccgagaucccgccguUGCGCGCCGGc -3' miRNA: 3'- ccaaGCCGCG-GACUac-------------AUGCGCGGCC- -5' |
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12178 | 5' | -56.8 | NC_003309.1 | + | 15329 | 0.66 | 0.645871 |
Target: 5'- --gUCGGCGa-UGGcUGcUGCGCGUCGGa -3' miRNA: 3'- ccaAGCCGCggACU-AC-AUGCGCGGCC- -5' |
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12178 | 5' | -56.8 | NC_003309.1 | + | 15420 | 0.66 | 0.656614 |
Target: 5'- uGUcCGGCGCCgGAacaccuuCGCGCCGa -3' miRNA: 3'- cCAaGCCGCGGaCUacau---GCGCGGCc -5' |
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12178 | 5' | -56.8 | NC_003309.1 | + | 43942 | 0.66 | 0.667336 |
Target: 5'- gGGUUCGGCuuguGCCaGAUG-AUGUccuGUCGGa -3' miRNA: 3'- -CCAAGCCG----CGGaCUACaUGCG---CGGCC- -5' |
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12178 | 5' | -56.8 | NC_003309.1 | + | 34979 | 0.66 | 0.682291 |
Target: 5'- --gUUGGCGaagcugCUGAUGgauauccagacauCGCGCCGGa -3' miRNA: 3'- ccaAGCCGCg-----GACUACau-----------GCGCGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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