miRNA: 3'- -GUUUCG---UCUCGCGUgCCAGc-------CGUGGa -5'
miRNA: 3'- -GUUUCGucUCGCGUGC-CA---GCCGUGGa -5'
miRNA: 3'- guuuCGU-CUCGCG-UGCCaGCCGUGGa -5'
miRNA: 3'- guuuCGUCUCGCGUGCcAGCCGUgga -5'
miRNA: 3'- -GUUUCGUCUCGCGUGCCAGCCGUGGA- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12179 | 5' | -57 | NC_003309.1 | + | 34389 | 0.67 | 0.539528 |
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Target: 5'- uCAAAGCcuuGGAGCGCGaGGUCaaggaacuGCGCCg -3' miRNA: 3'- -GUUUCG---UCUCGCGUgCCAGc-------CGUGGa -5' |
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| 12179 | 5' | -57 | NC_003309.1 | + | 15437 | 0.69 | 0.418127 |
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Target: 5'- uCGAGGU--AGCGCGCGaGUgucCGGCGCCg -3' miRNA: 3'- -GUUUCGucUCGCGUGC-CA---GCCGUGGa -5' |
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| 12179 | 5' | -57 | NC_003309.1 | + | 5068 | 0.69 | 0.408761 |
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Target: 5'- ---uGCAcGGGCGCgGCGGcCGGUGCCg -3' miRNA: 3'- guuuCGU-CUCGCG-UGCCaGCCGUGGa -5' |
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| 12179 | 5' | -57 | NC_003309.1 | + | 25761 | 0.69 | 0.390429 |
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Target: 5'- ---cGCGGAGCGCAUGaUCGGCGa-- -3' miRNA: 3'- guuuCGUCUCGCGUGCcAGCCGUgga -5' |
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| 12179 | 5' | -57 | NC_003309.1 | + | 46126 | 1.06 | 0.000895 |
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Target: 5'- uCAAAGCAGAGCGCACGGUCGGCACCUc -3' miRNA: 3'- -GUUUCGUCUCGCGUGCCAGCCGUGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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