Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12181 | 3' | -58.1 | NC_003309.1 | + | 27746 | 0.66 | 0.565434 |
Target: 5'- -aGaCGACGCGCCGccCGGCGcaucgccgaaaUCAGUCc -3' miRNA: 3'- ugCcGUUGCGCGGCa-GUCGC-----------AGUCAG- -5' |
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12181 | 3' | -58.1 | NC_003309.1 | + | 4183 | 0.66 | 0.559027 |
Target: 5'- -aGGCAGCGCGgaUGUCGuccgccagcucgaacGCGcCGGUCg -3' miRNA: 3'- ugCCGUUGCGCg-GCAGU---------------CGCaGUCAG- -5' |
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12181 | 3' | -58.1 | NC_003309.1 | + | 22640 | 0.66 | 0.55583 |
Target: 5'- gGCGGCGcuggaagcgccgggcACGCcgucgccaccggcuGCCGUgCAGUGUCGGa- -3' miRNA: 3'- -UGCCGU---------------UGCG--------------CGGCA-GUCGCAGUCag -5' |
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12181 | 3' | -58.1 | NC_003309.1 | + | 32391 | 0.66 | 0.554766 |
Target: 5'- aGCGGCGACGgcuCGCUG-CGGCGgccguGUCc -3' miRNA: 3'- -UGCCGUUGC---GCGGCaGUCGCagu--CAG- -5' |
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12181 | 3' | -58.1 | NC_003309.1 | + | 14122 | 0.66 | 0.553702 |
Target: 5'- uCGGCGACGUGCCagaucuucacaccGUCAGacaCGUacuucgccCAGUCg -3' miRNA: 3'- uGCCGUUGCGCGG-------------CAGUC---GCA--------GUCAG- -5' |
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12181 | 3' | -58.1 | NC_003309.1 | + | 17812 | 0.66 | 0.544158 |
Target: 5'- uCGGCGAuCGuCGCCGUguGCGcguUCGG-Cg -3' miRNA: 3'- uGCCGUU-GC-GCGGCAguCGC---AGUCaG- -5' |
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12181 | 3' | -58.1 | NC_003309.1 | + | 4988 | 0.66 | 0.52315 |
Target: 5'- aGCGGCuuccGCGCGuUCGaUCuGCG-CGGUCa -3' miRNA: 3'- -UGCCGu---UGCGC-GGC-AGuCGCaGUCAG- -5' |
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12181 | 3' | -58.1 | NC_003309.1 | + | 18780 | 0.67 | 0.491234 |
Target: 5'- gACGGCAagaucgccgugaaACGauCGCCGgCGGCGaUAGUCg -3' miRNA: 3'- -UGCCGU-------------UGC--GCGGCaGUCGCaGUCAG- -5' |
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12181 | 3' | -58.1 | NC_003309.1 | + | 43523 | 0.67 | 0.482136 |
Target: 5'- gGCGGCcaucGCGCGCaucagGUC-GCGgCAGUCu -3' miRNA: 3'- -UGCCGu---UGCGCGg----CAGuCGCaGUCAG- -5' |
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12181 | 3' | -58.1 | NC_003309.1 | + | 5060 | 0.67 | 0.462218 |
Target: 5'- cGCGGCGGCcgGUGCCGcgaCGGCgGUCGGa- -3' miRNA: 3'- -UGCCGUUG--CGCGGCa--GUCG-CAGUCag -5' |
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12181 | 3' | -58.1 | NC_003309.1 | + | 22454 | 0.67 | 0.452422 |
Target: 5'- cGCGGCGAuCGCacGCUGcCGGCGUCGa-- -3' miRNA: 3'- -UGCCGUU-GCG--CGGCaGUCGCAGUcag -5' |
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12181 | 3' | -58.1 | NC_003309.1 | + | 5462 | 0.67 | 0.452422 |
Target: 5'- cGCGGCcAUcuucuuCGCCGUCAGCuUCAGaUCg -3' miRNA: 3'- -UGCCGuUGc-----GCGGCAGUCGcAGUC-AG- -5' |
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12181 | 3' | -58.1 | NC_003309.1 | + | 7892 | 0.69 | 0.387238 |
Target: 5'- uCGGCAa---GCCGaUCGGCGUCGGUg -3' miRNA: 3'- uGCCGUugcgCGGC-AGUCGCAGUCAg -5' |
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12181 | 3' | -58.1 | NC_003309.1 | + | 48835 | 0.69 | 0.387238 |
Target: 5'- gACGcuuCAGCGuCGCCGUUcGCGUCAGg- -3' miRNA: 3'- -UGCc--GUUGC-GCGGCAGuCGCAGUCag -5' |
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12181 | 3' | -58.1 | NC_003309.1 | + | 43780 | 0.69 | 0.369781 |
Target: 5'- cCGGCAAacgucucucCGCGCa-UCGGCGUCuGUCc -3' miRNA: 3'- uGCCGUU---------GCGCGgcAGUCGCAGuCAG- -5' |
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12181 | 3' | -58.1 | NC_003309.1 | + | 53265 | 0.69 | 0.369781 |
Target: 5'- aACcGCucGCGCGCCGUCuucgccGCGUgaCAGUCa -3' miRNA: 3'- -UGcCGu-UGCGCGGCAGu-----CGCA--GUCAG- -5' |
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12181 | 3' | -58.1 | NC_003309.1 | + | 44180 | 0.69 | 0.361257 |
Target: 5'- cGCGGC-GCGCuGCCGcUCGGCGUacgcgacgAGUCc -3' miRNA: 3'- -UGCCGuUGCG-CGGC-AGUCGCAg-------UCAG- -5' |
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12181 | 3' | -58.1 | NC_003309.1 | + | 10952 | 0.72 | 0.254608 |
Target: 5'- uACGcGCGA-GCGCCGUCuGCGccgucgccgcgcuuUCAGUCg -3' miRNA: 3'- -UGC-CGUUgCGCGGCAGuCGC--------------AGUCAG- -5' |
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12181 | 3' | -58.1 | NC_003309.1 | + | 37583 | 0.74 | 0.19284 |
Target: 5'- uACGGCGACGa-CCG-CGGCGUCAacGUCg -3' miRNA: 3'- -UGCCGUUGCgcGGCaGUCGCAGU--CAG- -5' |
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12181 | 3' | -58.1 | NC_003309.1 | + | 11973 | 0.74 | 0.187792 |
Target: 5'- cGCGGC-GCGCGCCGcgUCGGCGUUgaAGcCg -3' miRNA: 3'- -UGCCGuUGCGCGGC--AGUCGCAG--UCaG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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