Results 21 - 23 of 23 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 12181 | 3' | -58.1 | NC_003309.1 | + | 32391 | 0.66 | 0.554766 |
|
Target: 5'- aGCGGCGACGgcuCGCUG-CGGCGgccguGUCc -3' miRNA: 3'- -UGCCGUUGC---GCGGCaGUCGCagu--CAG- -5' |
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| 12181 | 3' | -58.1 | NC_003309.1 | + | 22640 | 0.66 | 0.55583 |
|
Target: 5'- gGCGGCGcuggaagcgccgggcACGCcgucgccaccggcuGCCGUgCAGUGUCGGa- -3' miRNA: 3'- -UGCCGU---------------UGCG--------------CGGCA-GUCGCAGUCag -5' |
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| 12181 | 3' | -58.1 | NC_003309.1 | + | 4183 | 0.66 | 0.559027 |
|
Target: 5'- -aGGCAGCGCGgaUGUCGuccgccagcucgaacGCGcCGGUCg -3' miRNA: 3'- ugCCGUUGCGCg-GCAGU---------------CGCaGUCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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