Results 21 - 22 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12182 | 3' | -58.8 | NC_003309.1 | + | 48116 | 0.68 | 0.400432 |
Target: 5'- cGCUCGCGCUuacuGUCGAacuugauGCCGuCGUGcUCg -3' miRNA: 3'- aCGAGCGUGGu---CAGCU-------CGGU-GCGC-AG- -5' |
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12182 | 3' | -58.8 | NC_003309.1 | + | 53262 | 0.67 | 0.448166 |
Target: 5'- cGCUCGCGCgCcGUCuucGCCGCGUGa- -3' miRNA: 3'- aCGAGCGUG-GuCAGcu-CGGUGCGCag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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