miRNA: 3'- gucagCCCGUCGAGCUaa--CUAGCGuc -5'
miRNA: 3'- guCAGCCCGUCgA-GCUaacuaGCGUC- -5'
miRNA: 3'- -GUCAGcCCGUCGAGCUAacuAGCGuc -5'
miRNA: 3'- -GUCAGCCCGUCGAGCUAACUAGCGUC- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12182 | 5' | -54.3 | NC_003309.1 | + | 9298 | 0.66 | 0.722449 |
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Target: 5'- ----aGGGCGGCUUGAaacgGAUCGCc- -3' miRNA: 3'- gucagCCCGUCGAGCUaa--CUAGCGuc -5' |
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| 12182 | 5' | -54.3 | NC_003309.1 | + | 43593 | 0.67 | 0.678664 |
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Target: 5'- -cGUCGGGCAGgUaCGAg----CGCAGg -3' miRNA: 3'- guCAGCCCGUCgA-GCUaacuaGCGUC- -5' |
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| 12182 | 5' | -54.3 | NC_003309.1 | + | 245 | 0.72 | 0.421957 |
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Target: 5'- cCAGUCcGGCGGCUCGAUgu-UCGUg- -3' miRNA: 3'- -GUCAGcCCGUCGAGCUAacuAGCGuc -5' |
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| 12182 | 5' | -54.3 | NC_003309.1 | + | 47386 | 1.09 | 0.001231 |
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Target: 5'- aCAGUCGGGCAGCUCGAUUGAUCGCAGu -3' miRNA: 3'- -GUCAGCCCGUCGAGCUAACUAGCGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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