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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12185 | 5' | -49 | NC_003309.1 | + | 13684 | 0.67 | 0.938513 |
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Target: 5'- aGACGgcgccauuCUUGAUCGGCUCGg -3' miRNA: 3'- gCUGCagaacaacGAACUAGUCGAGC- -5' |
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| 12185 | 5' | -49 | NC_003309.1 | + | 10362 | 0.67 | 0.930701 |
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Target: 5'- gCGcCGcCUcgUGUUGCUUGAUCGucuccucgacgagcGCUCGc -3' miRNA: 3'- -GCuGCaGA--ACAACGAACUAGU--------------CGAGC- -5' |
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| 12185 | 5' | -49 | NC_003309.1 | + | 33823 | 0.71 | 0.772758 |
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Target: 5'- uCGACGUgUucgcccgccguauUGUUgGCUggcgGGUCAGCUCGu -3' miRNA: 3'- -GCUGCAgA-------------ACAA-CGAa---CUAGUCGAGC- -5' |
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| 12185 | 5' | -49 | NC_003309.1 | + | 1197 | 0.72 | 0.752456 |
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Target: 5'- gCGcCGUCgccuggGUUGCcgaUGAUCGGCUCa -3' miRNA: 3'- -GCuGCAGaa----CAACGa--ACUAGUCGAGc -5' |
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| 12185 | 5' | -49 | NC_003309.1 | + | 48155 | 1.11 | 0.003311 |
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Target: 5'- cCGACGUCUUGUUGCUUGAUCAGCUCGa -3' miRNA: 3'- -GCUGCAGAACAACGAACUAGUCGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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