Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12186 | 3' | -52 | NC_003309.1 | + | 5203 | 0.69 | 0.727637 |
Target: 5'- aUCGCgacuucUUCGccGAAGCCGCG-CUCGAu -3' miRNA: 3'- gAGCG------AAGCucCUUUGGCGUaGAGCU- -5' |
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12186 | 3' | -52 | NC_003309.1 | + | 17833 | 0.71 | 0.605262 |
Target: 5'- aUCGCUUCGAuGuuGAUCGCGUCggCGAu -3' miRNA: 3'- gAGCGAAGCU-CcuUUGGCGUAGa-GCU- -5' |
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12186 | 3' | -52 | NC_003309.1 | + | 42433 | 0.7 | 0.672559 |
Target: 5'- aUCGacuUUCGAacGGugagccacuGAACCGCGUCUCGAa -3' miRNA: 3'- gAGCg--AAGCU--CC---------UUUGGCGUAGAGCU- -5' |
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12186 | 3' | -52 | NC_003309.1 | + | 49690 | 1.1 | 0.002067 |
Target: 5'- cCUCGCUUCGAGGAAACCGCAUCUCGAu -3' miRNA: 3'- -GAGCGAAGCUCCUUUGGCGUAGAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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